"
Team:WPI-Worcester/Overview
From 2014.igem.org
(Difference between revisions)
Cllajeunesse (Talk | contribs) |
Cllajeunesse (Talk | contribs) |
||
| Line 4: | Line 4: | ||
<meta name="generator" content="MediaWiki 1.16.5" /> | <meta name="generator" content="MediaWiki 1.16.5" /> | ||
<link rel="shortcut icon" href="/favicon.ico" /> | <link rel="shortcut icon" href="/favicon.ico" /> | ||
| - | <link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title=" | + | <link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2014.igem.org (en)" /> |
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:WPI-Worcester&action=creativecommons" rel="meta" /> | <link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:WPI-Worcester&action=creativecommons" rel="meta" /> | ||
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" /> | <link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" /> | ||
| - | <link rel="alternate" type="application/atom+xml" title=" | + | <link rel="alternate" type="application/atom+xml" title="2014.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&feed=atom" /> <title>Team:WPI-Worcester - 2014.igem.org</title> |
<style type="text/css" media="screen, projection">/*<![CDATA[*/ | <style type="text/css" media="screen, projection">/*<![CDATA[*/ | ||
@import "/wiki/skins/common/shared.css?270"; | @import "/wiki/skins/common/shared.css?270"; | ||
| Line 30: | Line 30: | ||
wgVariantArticlePath=false, | wgVariantArticlePath=false, | ||
wgActionPaths={}, | wgActionPaths={}, | ||
| - | wgServer="https:// | + | wgServer="https://2014.igem.org", |
wgCanonicalNamespace="", | wgCanonicalNamespace="", | ||
wgCanonicalSpecialPageName=false, | wgCanonicalSpecialPageName=false, | ||
| Line 51: | Line 51: | ||
wgDigitTransformTable=["", ""], | wgDigitTransformTable=["", ""], | ||
wgMainPageTitle="Main Page", | wgMainPageTitle="Main Page", | ||
| - | wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": " | + | wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2014.igem.org", "5": "2014.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"}, |
| - | wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, " | + | wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2014.igem.org": 4, "2014.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7}, |
| - | wgSiteName=" | + | wgSiteName="2014.igem.org", |
wgCategories=[], | wgCategories=[], | ||
| - | wgMWSuggestTemplate="https:// | + | wgMWSuggestTemplate="https://2014.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest", |
| - | wgDBname=" | + | wgDBname="2014_igem_org", |
wgSearchNamespaces=[0], | wgSearchNamespaces=[0], | ||
wgMWSuggestMessages=["with suggestions", "no suggestions"], | wgMWSuggestMessages=["with suggestions", "no suggestions"], | ||
| Line 91: | Line 91: | ||
<div id="search-controls" class="noprint"> | <div id="search-controls" class="noprint"> | ||
<form action="/Special:Search" id="searchform"> | <form action="/Special:Search" id="searchform"> | ||
| - | <input id="searchInput" name="search" type="text" title="Search | + | <input id="searchInput" name="search" type="text" title="Search 2014.igem.org [f]" accesskey="f" value="" /> |
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" /> | <input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" /> | ||
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /> | <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /> | ||
| Line 101: | Line 101: | ||
<h1 class="firstHeading">Team:WPI-Worcester</h1> | <h1 class="firstHeading">Team:WPI-Worcester</h1> | ||
<div id="bodyContent"> | <div id="bodyContent"> | ||
| - | <h3 id="siteSub" class='noprint'>From | + | <h3 id="siteSub" class='noprint'>From 2014.igem.org</h3> |
<div id="contentSub"></div> | <div id="contentSub"></div> | ||
<!-- | <!-- | ||
| Line 600: | Line 600: | ||
<ul> | <ul> | ||
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Survey">Survey</a></li> | <li><a href="https://2014.igem.org/Team:WPI-Worcester/Survey">Survey</a></li> | ||
| - | <li><a href="https:// | + | <li><a href="https://2014.igem.org/Team:WPI-Worcester/Outreach">Outreach</a></li> |
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Collaborations">Collaborations</a></li> | <li><a href="https://2014.igem.org/Team:WPI-Worcester/Collaborations">Collaborations</a></li> | ||
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Interlab">Interlab Study</a></li> | <li><a href="https://2014.igem.org/Team:WPI-Worcester/Interlab">Interlab Study</a></li> | ||
Revision as of 03:28, 16 October 2014
"
Team:WPI-Worcester
From 2014.igem.org
Overview
The goal of this project is to create a simple, cheap, fast-acting, and effective diagnostic tool that can be used to determine whether or not an organism is infected with any disease, as long as the antibody and antigen are known and available. CAEV was determined to be a good starting point based on the economic value of goats worldwide.
First, in the lab, the capsid protein p28 of CAEV is ligated onto the N-terminal domain of BclA, and a ribosome binding site, a constitutive promoter, and a double terminator are added to this construct. Next, competent E. coli are transformed with this plasmid, producing bacteria that express the CAEV p28 surface antigen on an even layer on the surface of its plasma membrane.
On the farm, a blood sample is taken from a goat to be tested for CAEV. The sample is transported to the lab, where it is centrifuged to separate out the plasma. Once the plasma is isolated from the pellet of blood cells, it is combined with the genetically engineered E. coli in a 96-well plate up to a titer of 5000.
If the goat is infected with CAEV, then the p28 antibodies should be present in its plasma. If the antibody is present, it will agglutinate with the E. coli expressing BclA and CAEV p28 when combined and left to rest overnight. This allows us to determine whether or not a goat is infected based on the results of a simple agglutination assay.
