Team:StanfordBrownSpelman/BioBricks

BioBricks

A major goal of the iGEM foundation is to allow for the black-boxing of DNA parts that can later be utilized and recombined innovatively to create new, useful things. These black-boxed DNA parts are called BioBricks. Each of our projects has contributed to the Registry of Standard Biological Parts.

Cellulose Acetate

Part BBa_K1499000: This part is for the wssF region of the wss operon isolated from Pseudomonas fluorescens. This operon is responsible for the acetylation of cellulose.

Part BBa_K1499001: This part is for the wssG region of the wss operon isolated from Pseudomonas fluorescens. This operon is responsible for the acetylation of cellulose.

Part BBa_K1499002: This part is for the wssH region of the wss operon isolated from Pseudomonas fluorescens. This operon is responsible for the acetylation of cellulose.

Part BBa_K1499003: This part is for the wssI region of the wss operon isolated from Pseudomonas fluorescens. This operon is responsible for the acetylation of cellulose.

Cellulose Cross Linker

Part BBa_K1499004: This part encodes the expression of a cellulose cross linking protein. It contains two different cellulose binding domains taken from species Clostridium cellulovorans, and a streptavidin domain in between that allows living cells expressing a biotinylated AviTag to attach to the cellulose.

Amberless Hell Cell

Part BBa_K1499200: uvsE

Part BBa_K1499201: uracil glycosylase 1

Part BBa_K1499202: uracil glycosylase 2

Part BBa_K1499203: SdaB protein generator without tRNA + 5 amber stops

Part BBa_K1499205: MntH with amber stop codon with promoter for expression, no tRNA

Part BBa_K1499250: GFP (E0040) with two amber stop codons

Part BBa_K1499251: tRNA supP

Part BBa_K1499252: GFP generator with supP

Part BBa_K1499253: aeBlue generator with 3 UAG stops + tRNA

Material Waterproofing

Part BBa_K1499400: This is one version of a chitin binding proteins found in the Polistes dominula saliva that may be responsible for the waterproofing capability of cellulose seen in paper wasp nests.

Part BBa_K1499401: This is another version of a chitin binding proteins found in the Polistes dominula saliva that may be responsible for the waterproofing capability of cellulose seen in paper wasp nests.

Part BBa_K1499402: This is a completely uncharacterized protein from the Polistes dominula transcriptome that may be a major player in the paper wasp's ability to waterproof cellulose.

Biodegradability

Part BBa_K1499500: This is a part that encodes quorum sensing machinery to activate GFP expression. It is fundamentally a combination of two parts, BBa_I13202 and BBa_T9002.

Part BBa_K1499501: This is a part that encodes the endo-1,4-beta-glucanase, or cellulase gene. This protein is a means of breaking down cellulose and is is specific for 1,4-beta linkages in cellulose. It was isolated from Neisseria sicca.

Part BBa_K1499503: This part is the same as Part BBa_K1499500 but has two more terminators between the luxR gene and the luxPR promoter.

      

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