Team:UFAM Brazil/7-10-2014

From 2014.igem.org

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The colonies from the transformation in <i>Escherichia coli</i> DH5α and RR1 with the Biobrick Transformation Efficiency Kit as a control and the DNA diluted from the 2013 DNA Part Kit (BBa_E0840) greeeew!!!!  Although we were not sure if we could trust in the results. We made an inoculum using  liquid LB medium from the transformed cells (BBa_E0840 and Transformation Efficiency Kit) to make a miniprep  and check the electrophoretical profile from the transformation. To get more satisfactory results we are going to repeat the transformation process with only the lining RR1, shown in the following :
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The transformed colonies of <i>Escherichia coli</i> DH5α and RR1 with Biobrick Transformation Efficiency Kit as control and the diluted DNA from the 2013 DNA Part Kit (BBa_E0840) greeeew!!!!  Although we were not sure if we could trust in the results. We made an inoculum using  liquid LB medium from transformed cells (BBa_E0840 and Transformation Efficiency Kit) to make a miniprep  and check the electrophoretical profile of the transformation. To get more satisfactory results we are going to repeat the transformation process only with lineage RR1, shown next :
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<p style="margin-left:50px">- BBa_K346004 (Bioaccumulation Biobrick).</p>
<p style="margin-left:50px">- BBa_K346004 (Bioaccumulation Biobrick).</p>
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<p style="margin-top:20px">We have followed iGEM’s Transformation Protocol.  LB plates – agar with 20ug/ml of chloranphenicol.</p>
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<p style="margin-top:20px">We followed iGEM’s Transformation Protocol.  LB plates – agar with 20ug/ml of chloranphenicol.</p>
<p> Waiting for our synthesis to arrive!!</p>
<p> Waiting for our synthesis to arrive!!</p>
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Latest revision as of 23:26, 16 October 2014

07/10/2014

The transformed colonies of Escherichia coli DH5α and RR1 with Biobrick Transformation Efficiency Kit as control and the diluted DNA from the 2013 DNA Part Kit (BBa_E0840) greeeew!!!! Although we were not sure if we could trust in the results. We made an inoculum using liquid LB medium from transformed cells (BBa_E0840 and Transformation Efficiency Kit) to make a miniprep and check the electrophoretical profile of the transformation. To get more satisfactory results we are going to repeat the transformation process only with lineage RR1, shown next :

- Negative control - no DNA;

- Positive control from the Transformation Efficiency Kit;

- BBa_E0840 (Biodetection Biobrick);

- BBa_K346004 (Bioaccumulation Biobrick).

We followed iGEM’s Transformation Protocol. LB plates – agar with 20ug/ml of chloranphenicol.

Waiting for our synthesis to arrive!!

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