Team:Valencia UPV/VUPV Parts

From 2014.igem.org

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<div align="center"><span class="coda"><roja>P</roja>arts</span> </div><br/>
<div align="center"><span class="coda"><roja>P</roja>arts</span> </div><br/>
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<p>Our team sent 7 BioBrick Parts to the <a class="normal-link-page" href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=Valencia_UPV">Registry</a>.</p><br/>
<p>Our team sent 7 BioBrick Parts to the <a class="normal-link-page" href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=Valencia_UPV">Registry</a>.</p><br/>
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<p>Four of them could be sent thanks to the collaboration from the <a class="normal-link-page" href="https://2014.igem.org/Team:NRP-UEA-Norwich">NRP-UEA-Norwich</a> team, who very kindly provided us with their MoFlippers. These were the parts coding sequences for <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554001">AtrΔ11</a>, <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554002">HarFAR</a> and <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554003">EaDAcT</a> and the <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554000">TA29</a> promoter.</p><br/>
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<p>We also designed a vector that has de backbone of the Biobrick vector pSB1A3 but compatible with GoldenBraid 2.0. It contains two BsmBI restriction sites which leave the required overhangs to perform a GoldenBraid ligation of two transcriptional units in alpha vectors.  We call it <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554006">"omega undercover"</a> as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone.</p><br/>
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<div align="center"><groupparts>iGEM014 Valencia_UPV</groupparts></div>
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<div align="center"><img src="https://static.igem.org/mediawiki/2014/4/45/VUPVomega_undercover.jpg" alt="omega_undercover"></img></div><br/>
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<p>Using the omega undercover plasmid, we constructed the two biosafety devices which consist on an identity preservation module, expressing either <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554004">yellow</a> or <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554005">blue</a> chromoprotein, and a male sterility module.</p><br/>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554000">BBa_K1554000</a> – Ta29 Promoter</b></p>
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<p>The Ta29 promoter is anther-specific. Anthers are the male organs of the flower, and their function is to produce pollen. A cytotoxic gene (barnase, ipt, RIP...) is usually used under the control of this specific promoter leading to a plant unable to produce pollen, inducing male-sterility. (see Biosafety)</p><br/>
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<div align="center"><img src="https://static.igem.org/mediawiki/2014/e/e2/VUPV_biosafety_constr_omega_undercover.jpg" alt="omega_undercover2"></img></div><br/><br/><br/><br/>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554001">BBa_K1554001</a> – AtrΔ11</b></p>
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<p>The AtrΔ11 protein is a delta-11-desaturase from Amyelois transitella that introduces an unsaturation between C11 and C12 in long-chain fatty acids. This DNA sequence underwent codon usage optimization for Nicotiana benthamiana.
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Acyl-CoA + Reduced acceptor + O2 = Delta11-acyl-CoA + Acceptor + 2 H2O
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This part was used in the Insect sexual pheromone production pathway (link Biosynthesis)</p><br/>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554002">BBa_K1554002</a> – HarFAR</b></p>
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<p>The HarFAR protein is a fatty acid reductase that catalyses the conversion of a long-chain fatty acid carboxyl group to an alcohol group. The DNA sequence was obtained from Helicoverpa armigera HarFAR-3 protein, including an Endoplasmic Reticulum retention signal (KKYR) in the C-terminal end. In addition, codon usage optimization was performed for Nicotiana benthamiana.
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This part was used in the Insect sexual pheromone production pathway (link Biosynthesis)</p><br/>
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<div align="center"><groupparts>iGEM014 Valencia_UPV</groupparts></div>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554003">BBa_K1554003</a> – EaDAcT</b></p>
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<p>The EaDAcT protein is a diacylglycerol acetyltransferase coming from Euonymus alatus. This enzyme can transform alcohol gropus from fatty acid to aldehyde gropus. This DNA sequence underwent codon usage optimization for Nicotiana benthamiana.
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This part was used in the Insect sexual pheromone production pathway (link Biosynthesis)</p><br/>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554004">BBa_K1554004</a> – Yellow biosafety module for plants</b></p>
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<p>This biosafety device for plants consists of two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consists of a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the yellow chromoprotein AmilGFP, so that plants can be visually differentiated from non-transgenic plants.</p><br/>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554005">BBa_K1554005</a> - Blue biosafety module for plants</b></p>
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<p>This biosafety device is composed of the same parts as the previously explained one, BBa_K1554004. This device uses AmilCP as chromoprotein instead of AmilGFP.</p><br/>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554006">BBa_K1554006</a> - Omega Undercover</b></p>
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<p>The Omega undercover vector allows the conversión of GoldenBraid standard parts to BioBrick’s. Methodology: Parts contruction</p><br/>
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Revision as of 22:07, 17 October 2014

Achievements > Parts


Parts

Our team sent 7 BioBrick Parts to the Registry.


<groupparts>iGEM014 Valencia_UPV</groupparts>


BBa_K1554000 – Ta29 Promoter

The Ta29 promoter is anther-specific. Anthers are the male organs of the flower, and their function is to produce pollen. A cytotoxic gene (barnase, ipt, RIP...) is usually used under the control of this specific promoter leading to a plant unable to produce pollen, inducing male-sterility. (see Biosafety)


BBa_K1554001 – AtrΔ11

The AtrΔ11 protein is a delta-11-desaturase from Amyelois transitella that introduces an unsaturation between C11 and C12 in long-chain fatty acids. This DNA sequence underwent codon usage optimization for Nicotiana benthamiana. Acyl-CoA + Reduced acceptor + O2 = Delta11-acyl-CoA + Acceptor + 2 H2O This part was used in the Insect sexual pheromone production pathway (link Biosynthesis)


BBa_K1554002 – HarFAR

The HarFAR protein is a fatty acid reductase that catalyses the conversion of a long-chain fatty acid carboxyl group to an alcohol group. The DNA sequence was obtained from Helicoverpa armigera HarFAR-3 protein, including an Endoplasmic Reticulum retention signal (KKYR) in the C-terminal end. In addition, codon usage optimization was performed for Nicotiana benthamiana. This part was used in the Insect sexual pheromone production pathway (link Biosynthesis)


BBa_K1554003 – EaDAcT

The EaDAcT protein is a diacylglycerol acetyltransferase coming from Euonymus alatus. This enzyme can transform alcohol gropus from fatty acid to aldehyde gropus. This DNA sequence underwent codon usage optimization for Nicotiana benthamiana. This part was used in the Insect sexual pheromone production pathway (link Biosynthesis)


BBa_K1554004 – Yellow biosafety module for plants

This biosafety device for plants consists of two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consists of a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the yellow chromoprotein AmilGFP, so that plants can be visually differentiated from non-transgenic plants.


BBa_K1554005 - Blue biosafety module for plants

This biosafety device is composed of the same parts as the previously explained one, BBa_K1554004. This device uses AmilCP as chromoprotein instead of AmilGFP.


BBa_K1554006 - Omega Undercover

The Omega undercover vector allows the conversión of GoldenBraid standard parts to BioBrick’s. Methodology: Parts contruction







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