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Team:AMU-Poznan/Modeling
From 2014.igem.org
(Difference between revisions)
| Line 87: | Line 87: | ||
<h3>Conclusions</h3> | <h3>Conclusions</h3> | ||
</br> | </br> | ||
| + | Knowledge of RNAi is good. however people do not know what exactly sh-miR is.</br> | ||
| + | Users are eager to wait up to 8h, 24h only 50% and 2 days only 20% (work without software will take 2 days).</br> | ||
| + | What are the most important features of bioinformatic software:</br> | ||
| + | user-friendly interface</br> | ||
| + | documentation</br> | ||
| + | easy user-guide</br> | ||
| + | fast response from software</br></br> | ||
| + | what is important in shmiR designer:</br> | ||
| + | speed of software</br> | ||
| + | possibilty to choose miRNA scaffold</br> | ||
| + | experimental evaluation</br> | ||
</br></br></br> | </br></br></br> | ||
<h1>Modeling</h1> | <h1>Modeling</h1> | ||
Revision as of 18:01, 15 October 2014
Survey14.10.2014 31 survey responses: what survey answerers think that RNAi is 
what they think that sh-miRs are
what they think that shRNAs are
what literature do they know about sh-miRs
what they find important in bioinformatic software
what they find important in sh-miR designer
how long will they wait for software response
survey answerers
their sex
and age
ConclusionsKnowledge of RNAi is good. however people do not know what exactly sh-miR is. Users are eager to wait up to 8h, 24h only 50% and 2 days only 20% (work without software will take 2 days). What are the most important features of bioinformatic software: user-friendly interface documentation easy user-guide fast response from software what is important in shmiR designer: speed of software possibilty to choose miRNA scaffold experimental evaluationModelingWe decided to pay more attention to model sh-miR molecules with different RNA 2D structure prediction software (we use RNAfold in our software). Here are our results: analyzed sequence: CTAAAGAAGGTATATTGCTGTTGACAGTGAGCGACTGUUUGUAUUCGCCCUAGCGCCTGTGAAGCCACAGATGGGGCGCUAUGGCGAAUACAAACATGCCTACTGCCTCGGACTTCAAGGGGCTACTTTAGGAGCA We always take first generated structure made with default options If there is difference in algoruthm we always take RNA folding over DNA folding mfold
RNAstructure
RNAfold
???
Conclusions
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