Team:UCLA/Notebook/Protocols

From 2014.igem.org

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<tr><td><br><b>Equipment for the collection of recombinant silk protocols</b></br><a href="https://static.igem.org/mediawiki/2014/a/ab/Equipment_Protocol.pdf">Equipment Protocol</a></td></tr>
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<tr><td><br><b>Equipment for the collection of recombinant silk</b></br><a href="https://static.igem.org/mediawiki/2014/a/ab/Equipment_Protocol.pdf">Equipment Protocol</a></td></tr>
<tr><td><br> <b>List of Reagents</b></br>
<tr><td><br> <b>List of Reagents</b></br>

Revision as of 19:52, 2 July 2014

iGEM UCLA




Protocols

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.


Equipment for the collection of recombinant silk
Equipment Protocol

List of Reagents
Reagent Protocol

PCR for MASP1 and MASP2
PCR Protocol

Silk Fibroin Extraction
Silk Fibroin Extraction Protocol

Silk Films
Silk Films Protocol

Silk Films
Electrospun fibers


Reference

Materials Fabrication
Rockwood, Danielle N., Rucsanda C. Preda, Tuna Yücel, Xiaoqin Wang, Michael L. Lovett, and David L. Kaplan. "Materials Fabrication from Bombyx Mori Silk Fibroin." Nature Protocols 6.10 (2011): 1612-631. Web.


Production of MASP1/2
Teulé, Florence, Alyssa R. Cooper, William A. Furin, Daniela Bittencourt, Elibio L. Rech, Amanda Brooks, and Randolph V. Lewis. "A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning." Nature Protocols 4.3 (2009): 341-55. Web.