Team:WashU StLouis

From 2014.igem.org

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Revision as of 20:16, 1 July 2014



Welcome!
We are Team NitroGENIUS!

Scroll down to find more about our project.

Nitrogen Fixation in E. coli

Two Key Components to our Project:

Our team is using genes from cyanobacteria to get nitrogen fixation working in E. coli and is working to regulate the transcription of these nitrogen fixation genes with light
Top

How does your project work?

Right now, our plan is to divide and conquer. Ben and Jeffrey are working in Dr. Tae Seok Moon's lab with Cheryl Immethun on the transcription regulation project while Richard and Caroline are working in Dr. Himadri Pakrasi's lab with Dr. Deng Liu, Bert Berla, and Andrew Ng on getting the cyanobacterial nif cluster to function in E. coli . We are looking to study how these nitrogen fixation genes function in a foreign environment and to generate a light-senstive transcription system; our end goal is to unite these two projects to create a system that fixes nitrogen only in the absence of light.

Top

Who will your project help?

Our project is the first step of a much larger, much more complex endeavor. Nitrogen overabundance and nitrogen depletion are simultaneously big stumbling blocks in modern agriculture. The solution to both of these problems would be to endow plants themselves with the ability to fix nitrogen so that they could autonomously supply their own nitrogen for proteins, DNA, etc. We are taking the first step towards this ambitious goal by studying how the genes for nitrogen fixation from cyanobacteria work in different environments and constructing an artificial transcriptional system. We are currently working in E. coli because it is easy to engineer, but the next step would be to move into a cyanobacteria more closely related to chloroplasts. We hope that by making these initial steps that we may be helping to pave the way for future research that may put an end to world hunger.
Top

Why did you choose this project?

What motivated you to work on this project? Tell us what inspires you.
Top

Important links to keep

There are a few wiki requirements teams must follow:
  • All pages, images and files must be hosted on the 2014.igem.org server.
  • All pages must be created under the team’s name space.
  • As part of your documentation, keep the links from the menu to the left.
  • Do not use flash in wiki code.
  • The iGEM logo should be placed on the upper part of every page and should link to 2014.igem.org.

Visit the Wiki How To page for a complete list of requirements, tips and other useful information.

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