Biosafety

Overview

All of NEFU_China members completed safety training before the start of our project. The programs about safety training can be found here: http://dept.nefu.edu.cn/life/list/?227_1.html.(Chinese version). The Laboratory Biosafety Manual published by WHO has provided practical guidance for us. We work in a BSL1 lab equipped well to meet the safety criteria.

Q&A Section

• Q1. Please describe the chassis orgamisms you will be using in your project.

• 	Species name	Risk Group	Risk Group Source	Disease risk to humans?
  1	Synechococcus elongates PCC7942	1	http://www.atcc.org/Products/All/33912.aspx	No
  2	Bacillus subtilis	1	It’s widely spread in the environment.   And it is used in various areas and beneficial to human.	No
  3	E.coli (DH5α)	1	www.absa.org/riskgroups/bacteriasearch .php?genus=Escherichia	No
  4	E.coli (Rosetta/DE3 pLysS)	1	www.absa.org/riskgroups/bacteriasearch .php?genus=Escherichia	No

• Q2. List and describe all new parts you will be using in your project.

• 	Part name	Natural function of part	How did you acquire it?	How will you use it?
  1	BBa_K1509000	Coding SmtB	Using PCR to isolate it from Synechococcus elongates PCC7942	Bind and regulate smtO/P
  2	BBa_K1509001	A regulator which is affected by SmtB protein	Using PCR to isolate it from Synechococcus elongates PCC7942	Detect the heavy metal ions with part1
  3	BBa_K1509002	Mainly responsible for flocculent activity	Using PCR to isolate it from Bacillus   subtilis	Flocculate bacteria to collect nanocrystals
  4	BBa_K1509003	A strong Constitutive promoter	Synthesized by BGI Tech	regulate CDS7 gene expression

• Q3. Risks to the safety and health of team members or others working in the lab?
• We worked in a BSL1 lab and all of us had received the safety training. We have worked out an emergency package.
• Q4. What safety training have you received?
• In July, we completed Laboratory Biosafety Manual, Chemical/Fire/Electrical safety trainings before the start of our wet lab work. You can get more information here:http://dept.nefu.edu.cn/life/list/?227_1.html(Chinese version) If you have any questions, please contact us.
• Q5. Risks to the security through malicious misuse by individuals, groups, or countries?
• The biological materials we used have no menace and we have effective measures to prevent someone from misusing.
• Q6. If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?
• Our project includes special feature to reduce this risk. The flocculation module was designed to carry our project forward to commercial/industrial product.
• Q7. Does your country have national biosafety regulations or guidelines? If so, please provide a link to these regulations or guidelines if possible.
• Here you can see our national biosafety regulations and guidelines: http://www.stee.agri.gov.cn/biosafety(Chinese version) If you have any questions, please contact us.