Revision as of 11:08, 15 October 2014

Results / Heavy metals / Arabidopsis thaliana T4MBP

Transformation of A. thaliana with our T4MBP:

Exchange the original promoter enTCUP2 of the binary vector pORE_E3 (AY562536.1) into a 2x35s promoter using this protocol.
We integrated our T4MBP in our modified pORE_E3_2x35S.
Application of our floral dip method.
After 6 weeks we harvested transgenic seeds from A. thaliana and plated these seeds on selection MSO-media.
At last we potted transformed plants.

We achieved to regenerate young A. thaliana after the transformation with our T4MBP.

Fig. 1: Pictures showing young potentially transgenic A. thaliana before, during and after the transfer from medium to earth.

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 <h1>Results / Heavy metals / <i>Arabidopsis thaliana</i> T4MBP</h1>
-<h2>Labwork</h2><p class="text"><ul><li>exchange the original promoter enTCUP2 of the binary vector pORE_E3 <a href="http://www.ncbi.nlm.nih.gov/nuccore/AY562536">(AY562536.1)</a> into a 2x35s promoter using <a href="">this protocol</a></li></li><li><a href="" target="_blank">floral dip </a>method</li><ol><li>integrated our T4MBP in our modified pORE_E3_2x35S</li><li>floral dip</li><li>after 6 weeks we harvested transgenic seeds from <i>A. thaliana</i> and plated this seeds on selection MSO-media</li><li>potted transformed plants</li></ol></ul></p>
+<h2>Labwork</h2><p class="text">Transformation of <i>A. thaliana</i> with our T4MBP:<ol><li><b></b>Exchange the original promoter enTCUP2 of the binary vector pORE_E3 <a href="http://www.ncbi.nlm.nih.gov/nuccore/AY562536">(AY562536.1)</a> into a 2x35s promoter using <a href="">this protocol.</a></li><li>We integrated our T4MBP in our modified pORE_E3_2x35S.</li><li>Application of our <a href="">floral dip </a>method.</li><li>After 6 weeks we harvested transgenic seeds from <i>A. thaliana</i> and plated these seeds on selection MSO-media.</li><li>At last we potted transformed plants.</li></ol></p>
 <h2>Results</h2>