Revision as of 10:52, 15 October 2014

Results / Heavy metals / Arabidopsis thaliana T4MBP

exchange the original promoter enTCUP2 of the binary vector pORE_E3 (AY562536.1) into a 2x35s promoter using this protocol
floral dip method

integrated our T4MBP in our modified pORE_E3_2x35S
floral dip
after 6 weeks we harvested transgenic seeds from A. thaliana and plated this seeds on selection MSO-media
potted transformed plants

We achieved to regenerate young A. thaliana after the transformation with our T4MBP.

Fig. 1: Pictures showing young potentially transgenic A. thaliana before, during and after the transfer from medium to earth.

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 <h2>Results</h2>
-<p class="text">We achieved to regenerate young <i>A. thaliana</i> after the transformation them with our T4MBP.</p>
+<p class="text">We achieved to regenerate young <i>A. thaliana</i> after the transformation with our T4MBP.</p>
 <center><table border="0"><tr><td><a href="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141012_Arabudopsis-results1.jpg
 " data-lightbox="galery1" data-title="Fig. 1a: Young potentially transgenic <i>A. thaliana</i> during the transfer from medium to earth. "><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141012_Arabudopsis-results1.jpg" width="300px"></a></td><td><a href="https://static.igem.org/mediawiki/2014/1/13/Hannover_20141012_Arabudopsis-results3.jpg