Revision as of 10:51, 15 October 2014

Results / Heavy metals / Arabidopsis thaliana T4MBP

exchange the original promoter enTCUP2 of the binary vector pORE_E3 (AY562536.1) into a 2x35s promoter using this protocol
floral dip method

integrated our T4MBP in our modified pORE_E3_2x35S
floral dip
after 6 weeks we harvested transgenic seeds from A. thaliana and plated this seeds on selection MSO-media
potted transformed plants

We achieved to regenerated young A. thaliana after the transformation them with our T4MBP.

Fig. 1: Pictures showing young potentially transgenic A. thaliana before, during and after the transfer from medium to earth.

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 <h1>Results / Heavy metals / <i>Arabidopsis thaliana</i> T4MBP</h1>
-<h2>Labwork</h2><p class="text"><ul><li>floral dip method using<a href="" target="_blank"> our protocol</a></li><ol><li>integrated our T4MBP in our modified pORE_E3_2x35S</li><li>floral dip</li><li>after 6 weeks we harvested transgenic seeds from <i>A. thaliana</i> and plated this seeds on selection MSO-media</li><li>potted transformed plants</li></ol></ul></p>
+<h2>Labwork</h2><p class="text"><ul><li>exchange the original promoter enTCUP2 of the binary vector pORE_E3 <a href="http://www.ncbi.nlm.nih.gov/nuccore/AY562536">(AY562536.1)</a> into a 2x35s promoter using <a href="">this protocol</a></li></li><li><a href="" target="_blank">floral dip </a>method</li><ol><li>integrated our T4MBP in our modified pORE_E3_2x35S</li><li>floral dip</li><li>after 6 weeks we harvested transgenic seeds from <i>A. thaliana</i> and plated this seeds on selection MSO-media</li><li>potted transformed plants</li></ol></ul></p>
 <h2>Results</h2>