|
|
| Line 1: |
Line 1: |
| - |
| |
| | <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> | | <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> |
| | <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"> | | <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"> |
| Line 630: |
Line 629: |
| | </div> | | </div> |
| | </body> | | </body> |
| | + | <p>This is where content goes </p> |
| | | | |
| | | | |
| - | <br>
| |
| - | <head>
| |
| - | <br>
| |
| - | <h11><center>Fuse, or Die: The Case for the MoClo Revolution</center></h11>
| |
| - | </head>
| |
| - | <br><br>
| |
| - | <head>
| |
| - | <link href="http://static.tumblr.com/xrludau/Dyumrj1n1/js-image-slider.css" rel="stylesheet" type="text/css" />
| |
| - | <script src="http://static.tumblr.com/xrludau/3mXmrj1pd/js-image-slider.js" type="text/javascript"></script>
| |
| - | </head>
| |
| - | <body>
| |
| - | <span>
| |
| - | <div id="slider">
| |
| - | <a href="https://2014.igem.org/Team:WPI-Worcester">
| |
| - | <img src="https://static.igem.org/mediawiki/2013/1/1d/Fuse.png"></a>
| |
| - | <a href="https://2013.igem.org/Team:BostonU/MoCloChara">
| |
| - | <img src="https://static.igem.org/mediawiki/2013/b/b1/Slider_moclo.png"></a>
| |
| - | <a href="https://2013.igem.org/Team:BostonU/Data">
| |
| - | <img src="https://static.igem.org/mediawiki/2013/a/a1/Charslider.png"></a>
| |
| - | <a href="https://2013.igem.org/Team:BostonU/QS">
| |
| - | <img src="https://static.igem.org/mediawiki/2013/8/88/Slider_qs.png"></a>
| |
| - | <a href="https://2013.igem.org/Team:BostonU/DatasheetApp">
| |
| - | <img src="https://static.igem.org/mediawiki/2013/7/7a/Slider_ds.png"></a>
| |
| - | </div>
| |
| - | </span>
| |
| - | </body>
| |
| - |
| |
| - | <br><br>
| |
| - | <body>
| |
| - | <h7><p>Synthetic biology exists more as a form of art than a reproducible, well-defined production chain. From laboratory to laboratory, the experiments vary in procedure, characterization, and yield. The main product of synthetic biology — engineered organisms, are available only to the highly-experienced researcher and are not without the costs of timely preparation and low product yield. Consequently, the lack of standardization across the field has impeded the product from ever reaching a wide industry audience. More recent engineering efforts in the assembly of gene circuits has provided a pathway to a modular view of genetic parts. Termed the Modular Cloning Assembly Method (MoClo), this novel single-pot reaction protocol is a time-efficient, two-enzyme system for DNA assembly <a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0016765">(Weber et al., 2011)</a>. Before MoClo can reach its full potential across research and industry interests, the synthetic biology community needs a standardized and well-characterized library of MoClo parts for <i>Escherichia coli</i> to enable protocol automation and informed device designing. The 2013 Boston University iGEM Team seeks to bridge this gap in the product development chain by building a standard library and characterizing the parts via flow cytometry. To further efforts to develop foundational advancements for synthetic biology, we are taking a multi-faceted approach and working at several aspects and levels of design automation by:</p>
| |
| - | <p>
| |
| - | <ul>
| |
| - | <li>expanding a library of basic Level 0 MoClo parts by cloning from BioBrick parts and making new parts</li>
| |
| - | <li>building a library of composite Level 1 and 2 devices to characterize promoter-5' Untranslated Region combinations and demonstrate the library's usefulness</li>
| |
| - | <li>providing feedback on Clotho 2.0 software tools including the EugeneCAD language to a team of developers in the <a href="http://wiki.bu.edu/ece-cidar/index.php/Main_Page">CIDAR Lab</a></li>
| |
| - | <li>working with the <a href="https://2013.igem.org/Team:Wellesley_Desyne">Wellesley Desyne</a> team to develop an easy-to-use visualized programming language to wrap around Eugene</li>
| |
| - | <li>continuing the <a href="https://2012.igem.org/Team:BostonU/DataSheet">Datasheet project from the 2012 BostonU team</a> by finalizing a format for sharing information with <a href="https://2013.igem.org/Team:Purdue">Purdue Biomaker's iGEM Team</a> and programming a web app to generate the standardized datasheets</li>
| |
| - | </ul>
| |
| - | </p>
| |
| - | </h7>
| |
| - | </body>
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br>
| |
| - | <!--Twitter Link-->
| |
| - | <center><a href="https://twitter.com/BostonU_iGEM"><img src="https://static.igem.org/mediawiki/2013/d/da/Twitter-bird-blue-on-white.png" width=75></a></center>
| |
| - | <br>
| |
| - |
| |
| - | <h6><center>Our Sponsors</center></h6><br>
| |
| - | <center><img src="https://static.igem.org/mediawiki/2013/a/a9/Sponsorsbu.png" width="800px"></center>
| |
| - |
| |
| - | <!--Hit Counter-->
| |
| - | <div align='center'><img src='http://www.hit-counts.com/counter.php?t=MTI2MjEwMg==' border='0' alt='Free Hit Counter'></div>
| |
| - |
| |
| - |
| |
| - |
| |
| - | </p>
| |
| - | <!--
| |
| - | NewPP limit report
| |
| - | Preprocessor node count: 9/1000000
| |
| - | Post-expand include size: 40/2097152 bytes
| |
| - | Template argument size: 0/2097152 bytes
| |
| - | Expensive parser function count: 0/100
| |
| - | -->
| |
| - |
| |
| - | <!-- Saved in parser cache with key 2013_igem_org:pcache:idhash:837-0!1!0!!en!2!edit=0 and timestamp 20141014181044 -->
| |
| - | <div class="printfooter">
| |
| - | Retrieved from "<a href="https://2014.igem.org/Team:WPI-Worcester">https://2014.igem.org/Team:WPI-Worcester</a>"</div>
| |
| - | <div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
| |
| - | <div class="visualClear"></div>
| |
| - | </div>
| |
| - | </div>
| |
| - | <!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE -- RDR -->
| |
| - | <div class="visualClear"></div>
| |
| - | <div id='footer-box' class='noprint'>
| |
| - | <div id="footer">
| |
| - | <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div> <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div> <ul id="f-list">
| |
| - |
| |
| - |
| |
| - | <!-- Recentchanges is not handles well DEBUG -->
| |
| - | <li id="t-recentchanges"><a href="/Special:RecentChanges"
| |
| - | title='Recent changes'>Recent changes</a></li>
| |
| - |
| |
| - | <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:WPI-Worcester"
| |
| - | title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
| |
| - |
| |
| - | <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:WPI-Worcester"
| |
| - | title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
| |
| - |
| |
| - |
| |
| - |
| |
| - | <li id="t-specialpages"><a href="/Special:SpecialPages"
| |
| - | title="List of all special pages [q]" accesskey="q">Special pages</a>
| |
| - | </li>
| |
| - | <li><a href='/Special:Preferences'>My preferences</a></li>
| |
| - | </ul>
| |
| - | </div> <!-- close footer -->
| |
| - | <div id='footer'>
| |
| - | <ul id="f-list">
| |
| - |
| |
| - | <li id="t-print"><a href="/wiki/index.php?title=Team:WPI-Worcester&printable=yes"
| |
| - | title="Printable version of this page [p]" accesskey="p">Printable version</a>
| |
| - | </li>
| |
| - |
| |
| - | <li id="t-permalink"><a href="/wiki/index.php?title=Team:WPI-Worcester&oldid=241628"
| |
| - | title="Permanent link to this revision of the page">Permanent link</a>
| |
| - | </li>
| |
| - |
| |
| - |
| |
| - | <li id="privacy"><a href="/2013.igem.org:Privacy_policy" title="2013.igem.org:Privacy policy">Privacy policy</a></li>
| |
| - | <li id="disclaimer"><a href="/2013.igem.org:General_disclaimer" title="2013.igem.org:General disclaimer">Disclaimers</a></li>
| |
| - | </ul>
| |
| - | </div> <!-- close footer -->
| |
| - | </div> <!-- close footer-box -->
| |
| - |
| |
| - | <script>if (window.runOnloadHook) runOnloadHook();</script>
| |
| - | </div>
| |
| - | <!-- Served in 0.176 secs. --></body>
| |
| | | | |
| | </html> | | </html> |
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
"
"
