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Team:Heidelberg/pages/LOV/inductionBox
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A photo-induction box was constructed using blue LEDs (light emitting diodes) and foamboard obtained from garbage. | A photo-induction box was constructed using blue LEDs (light emitting diodes) and foamboard obtained from garbage. | ||
The device was designed such that a each well of a 24-well plate is fully covered by the light-conus of one LED. When using glass-bottom microscopy plates this should enable the simultaneous induction (or not-induction) at different intensities and an easy readout in one single flow cytometry assay. Alternatively, a separate plate can be wrapped in aluminium foil to keep it in the dark. | The device was designed such that a each well of a 24-well plate is fully covered by the light-conus of one LED. When using glass-bottom microscopy plates this should enable the simultaneous induction (or not-induction) at different intensities and an easy readout in one single flow cytometry assay. Alternatively, a separate plate can be wrapped in aluminium foil to keep it in the dark. | ||
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* Aluminium foil (to maximise irradiation through reflection inside the box and to prevent influence from the outside) | * Aluminium foil (to maximise irradiation through reflection inside the box and to prevent influence from the outside) | ||
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Revision as of 00:39, 15 October 2014
A photo-induction box was constructed using blue LEDs (light emitting diodes) and foamboard obtained from garbage.
The device was designed such that a each well of a 24-well plate is fully covered by the light-conus of one LED. When using glass-bottom microscopy plates this should enable the simultaneous induction (or not-induction) at different intensities and an easy readout in one single flow cytometry assay. Alternatively, a separate plate can be wrapped in aluminium foil to keep it in the dark.
General Data
- Wavelength: 460-470nm
- Format of cultures: Best used with 24-well plates
List of meterials used
- 24x LEDs from highlight-led.de (No. 75000001;50), LED 5mm wasserklar blau 12.000mcd 20°, 460-470nm 2.9-4.0V 20-30mA
- foambord (dark colour in order to prevent light from shining through)
- 96-well plate
- Aluminium foil (to maximise irradiation through reflection inside the box and to prevent influence from the outside)
