Team:Austin Texas/photocage
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After this exposure, the cells were grown for an additional [1 hour?], allowing the newly decaged T7 RNAP to transcribe the GFP reporter gene, which ultimately results in fluorescence. | After this exposure, the cells were grown for an additional [1 hour?], allowing the newly decaged T7 RNAP to transcribe the GFP reporter gene, which ultimately results in fluorescence. | ||
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+ | =References= | ||
+ | *Chou, C., Young, D. D. and Deiters, A. (2010), Photocaged T7 RNA Polymerase for the Light Activation of Transcription and Gene Function in Pro- and Eukaryotic Cells. ChemBioChem, 11: 972–977. doi: 10.1002/cbic.201000041 | ||
+ | *Deiters, A., Groff, D., Ryu, Y., Xie, J. and Schultz, P. G. (2006), A Genetically Encoded Photocaged Tyrosine. Angew. Chem., 118: 2794–2797. doi: 10.1002/ange.200600264 | ||
+ | *P. A. Osumidavis, N. Sreerama, D. B. Volkin, C. R. Middaugh, R. W. Woody, A. Y. M. Woody, J. Mol. Biol. 1994, J. Mol. Biol. 1994, 237, 5 – 19. | ||
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Revision as of 00:08, 15 October 2014
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