Team:Tuebingen/Notebook/Protocols/3A assembly

From 2014.igem.org

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<h3>Three part assembly</h3>
<h3>Three part assembly</h3>
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<h4><ul>Digestion</ul></h4>
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<h4><u>Digestion</u></h4>
<h4>Reagents</h4>
<h4>Reagents</h4>

Revision as of 23:19, 14 October 2014


Three part assembly

Digestion

Reagents

242 g Tris
57.1 mL pure acetic acid
0.5 M EDTA 0.5 M pH 8.0

Procedure

Mix all the reagents and add to 1000 ml with distillated water. Adjust to pH 8.3. Store at room temperature.

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