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Team:Tuebingen/Notebook/Protocols/PCR
From 2014.igem.org
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Revision as of 23:11, 14 October 2014
Polymerase chain reaction
Reagents
| Template (10-100 ng) | |
| 1 µL | Primer forward (10 µM) |
| 1 µL | Primer reverse (10 µM) |
| 1 µL | dNTP mix (10 mM) |
| 5 µL | 10x PCR buffer |
| 0.5 µL | Pfu Polymerase (1 U/µL) |
| to 50 µL | H20 |
Procedure
The reagents are mixed in a PCR-tube in which the polymerase is added last. Run the cycler using the appropriate program for the used polymerases and primers.
