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Team:Tuebingen/Notebook/Protocols/Ni NTA Buffers
From 2014.igem.org
(Difference between revisions)
| Line 12: | Line 12: | ||
<h3>Ni NA buffers</h3> | <h3>Ni NA buffers</h3> | ||
| - | < | + | <h4> Lysis buffer (pH = 8)</h4> |
<table border="0"> | <table border="0"> | ||
<colgroup> | <colgroup> | ||
| Line 37: | Line 37: | ||
<p> </p> | <p> </p> | ||
| - | < | + | <h4> Wash buffer (pH = 8)</h4> |
<table border="0"> | <table border="0"> | ||
<colgroup> | <colgroup> | ||
| Line 62: | Line 62: | ||
<p> </p> | <p> </p> | ||
| - | < | + | <h4> Elution buffer (pH = 8)</h4> |
<table border="0"> | <table border="0"> | ||
<colgroup> | <colgroup> | ||
Revision as of 22:49, 14 October 2014
Ni NA buffers
Lysis buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 10 mM | Immidazol |
Wash buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 20 mM | Immidazol |
Elution buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 250 mM | Immidazol |
