Team:Tuebingen/Notebook/Protocols/laemmli

From 2014.igem.org

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<h3>Laemmli buffer</h3>
<h3>Laemmli buffer</h3>
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<h4>Reagents</h4>
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<table border="0">
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  <colgroup>
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    <col width="80">
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    <col width="300">
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  </colgroup>
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  <tr>
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    <td style="text-align: center">277.8 mM</td>
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    <td>Tris-HCl, pH 6.8</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">4.4 %</td>
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    <td>SDS </td>
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  </tr>
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  <tr>
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    <td style="text-align: center">44.4% (w/v)</td>
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    <td>glycerol</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">0.02 %</td>
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    <td>bromophenol blue</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">10 % </td>
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    <td>β-Mercaptoethanol</td>
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  </tr>
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</table>
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</div>
</div>

Revision as of 22:04, 14 October 2014


Laemmli buffer

Reagents

277.8 mM Tris-HCl, pH 6.8
4.4 % SDS
44.4% (w/v) glycerol
0.02 % bromophenol blue
10 % β-Mercaptoethanol

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/laemmli"