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Team:Tuebingen/Notebook/Protocols/TBE buffer
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<h3>TBE buffer</h3> | <h3>TBE buffer</h3> | ||
| + | <h4>Reagents</h4> | ||
| + | <table border="0"> | ||
| + | <colgroup> | ||
| + | <col width="100"> | ||
| + | <col width="300"> | ||
| + | </colgroup> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">1 M</td> | ||
| + | <td>Tris</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">0.85 M</td> | ||
| + | <td>B(OH)3</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">1 M</td> | ||
| + | <td>EDTA 0.5 M pH 8.0</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <h4>Procedure</h4> | ||
| + | <p>Add the reagents to 1000 ml H20. Adjust to pH 8.3 with B(OH)3.</p> | ||
Revision as of 23:15, 14 October 2014
TBE buffer
Reagents
| 1 M | Tris |
| 0.85 M | B(OH)3 |
| 1 M | EDTA 0.5 M pH 8.0 |
Procedure
Add the reagents to 1000 ml H20. Adjust to pH 8.3 with B(OH)3.
