Team:BostonU/Data

From 2014.igem.org

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<h2>Tandem Promoter and Repressor Gene Primers</h2>
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<h7>
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<center>
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<table id="devices" style="border:1px solid black; border-collapse:collapse">
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  <tr>
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    <th style="border:1px solid black"><font color="#B22222">Device Name</font></th>
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    <th style="border:1px solid black"><font color="#B22222">Forward Primer</font></th>
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    <th style="border:1px solid black"><font color="#B22222">Sequence</font></th>
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    <th style="border:1px solid black"><font color="#B22222">Reverse Primer</font></th>
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    <th style="border:1px solid black"><font color="#B22222">Sequence</font></th>
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  </tr>
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  <tr>
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    <td style="border:1px solid black;">BetI_CD</td>
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    <td style="border:1px solid black;">BetI_For_C</td>
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    <td style="border:1px solid black;">ATGAAGACGTAATGGTGCCGAAACTGGGTATGCAGAGC</td>
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    <td style="border:1px solid black;">BetI_Rev_D</td>
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    <td style="border:1px solid black;">ACGAAGACCTACCTTTAATCGGTCGGCAGATGCTGGGT</td>
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  </tr>
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  <tr>
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    <td style="border:1px solid black;">PhlF_CD</td>
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    <td style="border:1px solid black;">PhlF_For_C</td>
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    <td style="border:1px solid black;">ATGAAGACGTAATGATGGCACGTACCCCGAGCCGTAGC</td>
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    <td style="border:1px solid black;">PhlF_Rev_D</td>
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    <td style="border:1px solid black;">ACGAAGACCTACCTTTAACGCTGTGTACCCGGACAAAC</td>
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  </tr>
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  <tr>
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    <td style="border:1px solid black;">BM3R1_CD</td>
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    <td style="border:1px solid black;">BM3R1_For_C</td>
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    <td style="border:1px solid black;">ATGAAGACGTAATGATGGAAAGCACCCCGACCAAACAG</td>
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    <td style="border:1px solid black;">BM3R1_Rev_D</td>
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    <td style="border:1px solid black;">ACGAAGACCTACCTTTAGCTCTGACGGCTCAGTGCTGC</td>
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  </tr>
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  <tr>
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    <td style="border:1px solid black;">LmrA_CD</td>
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    <td style="border:1px solid black;">LmrA_For_C</td>
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    <td style="border:1px solid black;">ATGAAGACGTAATGATGAGCTATGGTGATAGCCGTGAA</td>
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    <td style="border:1px solid black;">LmrA_Rev_D</td>
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    <td style="border:1px solid black;">ACGAAGACCTACCTTTAACGTTTCAGCAGATCCGGAAT</td>
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  </tr>
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    <tr>
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    <td style="border:1px solid black;">SrpR_CD</td>
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    <td style="border:1px solid black;"> SrpR_For_C </td>
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    <td style="border:1px solid black;"> ATGAAGACGTAATGATGGCACGTAAAACCGCAGCAGAA </td>
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    <td style="border:1px solid black;">SrpR_Rev_D</td>
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    <td style="border:1px solid black;">ACGAAGACCTACCTTTATTCGAAGGATTTCACCTGTTT</td>
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  </tr>
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  <tr>
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    <td style="border:1px solid black;">pTet_AK</td>
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    <td style="border:1px solid black;"> pTet_For_A </td>
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    <td style="border:1px solid black;">ATGAAGACGTGGAGtccctatcagtgatagagattgac</td>
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    <td style="border:1px solid black;">pTet_Rev_K</td>
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    <td style="border:1px solid black;">ACGAAGACCTGCATttcggtcagtgcgtcctgctgatg</td>
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  </tr>
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<tr>
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    <td style="border:1px solid black;">pTet_KB</td>
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    <td style="border:1px solid black;">pTet_For_K</td>
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    <td style="border:1px solid black;">ATGAAGACGTATGCtccctatcagtgatagagattgac</td>
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    <td style="border:1px solid black;">pTet_Rev_B</td>
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    <td style="border:1px solid black;">ACGAAGACCTAGTAttcggtcagtgcgtcctgctgatg</td>
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  </tr>
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  <tr>
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    <td style="border:1px solid black;">pBad_AK</td>
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    <td style="border:1px solid black;">pBad_For_A</td>
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    <td style="border:1px solid black;">ATGAAGACGTGGAGaagaaaccaattgtccatattgca</td>
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    <td style="border:1px solid black;">pBad_Rev_K</td>
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    <td style="border:1px solid black;">ACGAAGACCTGCATtatggagaaacagtagagagttgc</td>
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  </tr> 
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  <tr>
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    <td style="border:1px solid black;">pR10B2C80Gm_EF</td>
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    <td style="border:1px solid black;">pRepressible  with Fusion Protein </td>
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    <td style="border:1px solid black;">R0010_EB+BCD2_BC+C0080_CI+E0040m_ID+B0015_DF</td>
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    <td style="border:1px solid black;">DVL1_EF</td>
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    <td style="border:1px solid black;">Level 1</td>
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    <td style="border:1px solid black;">Kanamycin</td>
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    <td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1401614">BBa_K1401614</a></td>
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  </tr>
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  <tr>
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    <td style="border:1px solid black;">pR10B2C40Gm_EF</td>
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    <td style="border:1px solid black;">pRepressible with Fusion Protein </td>
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    <td style="border:1px solid black;">R0010_EB+BCD2_BC+C0040_CI+E0040m_ID+B0015_DF</td>
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    <td style="border:1px solid black;">DVL1_EF</td>
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    <td style="border:1px solid black;">Level 1</td>
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    <td style="border:1px solid black;">Kanamycin</td>
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    <td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1401615">BBa_K1401615</a></td>
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  </tr>
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  <tr>
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    <td style="border:1px solid black;">pJ00B2C12m_AE</td>
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    <td style="border:1px solid black;">pConstitutive</td>
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    <td style="border:1px solid black;">J23100_AB+BCD2_BC+C0012m_CD+B0015_DE</td>
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    <td style="border:1px solid black;">DVL1_AE</td>
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    <td style="border:1px solid black;">Level 1</td>
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    <td style="border:1px solid black;">Kanamycin</td>
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    <td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1401616">BBa_K1401616</a></td>
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  </tr>
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     <table width="100%" border="0" cellspacing="15" cellpadding="0">
     <table width="100%" border="0" cellspacing="15" cellpadding="0">
       <tr>
       <tr>

Revision as of 17:35, 15 October 2014



Data Collected

Tandem Promoter and Repressor Gene Primers

Device Name Forward Primer Sequence Reverse Primer Sequence
BetI_CD BetI_For_C ATGAAGACGTAATGGTGCCGAAACTGGGTATGCAGAGC BetI_Rev_D ACGAAGACCTACCTTTAATCGGTCGGCAGATGCTGGGT
PhlF_CD PhlF_For_C ATGAAGACGTAATGATGGCACGTACCCCGAGCCGTAGC PhlF_Rev_D ACGAAGACCTACCTTTAACGCTGTGTACCCGGACAAAC
BM3R1_CD BM3R1_For_C ATGAAGACGTAATGATGGAAAGCACCCCGACCAAACAG BM3R1_Rev_D ACGAAGACCTACCTTTAGCTCTGACGGCTCAGTGCTGC
LmrA_CD LmrA_For_C ATGAAGACGTAATGATGAGCTATGGTGATAGCCGTGAA LmrA_Rev_D ACGAAGACCTACCTTTAACGTTTCAGCAGATCCGGAAT
SrpR_CD SrpR_For_C ATGAAGACGTAATGATGGCACGTAAAACCGCAGCAGAA SrpR_Rev_D ACGAAGACCTACCTTTATTCGAAGGATTTCACCTGTTT
pTet_AK pTet_For_A ATGAAGACGTGGAGtccctatcagtgatagagattgac pTet_Rev_K ACGAAGACCTGCATttcggtcagtgcgtcctgctgatg
pTet_KB pTet_For_K ATGAAGACGTATGCtccctatcagtgatagagattgac pTet_Rev_B ACGAAGACCTAGTAttcggtcagtgcgtcctgctgatg
pBad_AK pBad_For_A ATGAAGACGTGGAGaagaaaccaattgtccatattgca pBad_Rev_K ACGAAGACCTGCATtatggagaaacagtagagagttgc
pR10B2C80Gm_EF pRepressible with Fusion Protein R0010_EB+BCD2_BC+C0080_CI+E0040m_ID+B0015_DF DVL1_EF Level 1 Kanamycin BBa_K1401614
pR10B2C40Gm_EF pRepressible with Fusion Protein R0010_EB+BCD2_BC+C0040_CI+E0040m_ID+B0015_DF DVL1_EF Level 1 Kanamycin BBa_K1401615
pJ00B2C12m_AE pConstitutive J23100_AB+BCD2_BC+C0012m_CD+B0015_DE DVL1_AE Level 1 Kanamycin BBa_K1401616

















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