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Team:BYU Provo/Notebook/CRISPR/julyaug
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| + | <p><u>7/07/2014 - Garrett Jensen.</u> | ||
| + | <br/>- Today I looked around for the M17 media that supposedly was delivered 2 weeks ago. Brother Lee and Dr. Robison both said that it was not in their lab but they are the only ones who signed out a package from Fischer Scientific 2 weeks ago. | ||
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| + | <p><u>7/09/2014 - Garrett Jensen.</u> | ||
| + | <br/>- Today I ran a PCR on the DNA that Skip isolated from S. thermophilus on June 26 using the universal 16S ribosomal primers. I used taq polymerase instead of Redtaq because the redtaq has been having some issues lately. If there is microbial DNA present then we may be able to get PCR for the CRISPR to work from it. In the meantime I am waiting for our M17 media to show up. | ||
| + | <br/></p> | ||
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Revision as of 22:28, 9 July 2014
BYU 2014 Notebook |
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7/07/2014 - Garrett Jensen.
- Today I looked around for the M17 media that supposedly was delivered 2 weeks ago. Brother Lee and Dr. Robison both said that it was not in their lab but they are the only ones who signed out a package from Fischer Scientific 2 weeks ago.
7/09/2014 - Garrett Jensen.
- Today I ran a PCR on the DNA that Skip isolated from S. thermophilus on June 26 using the universal 16S ribosomal primers. I used taq polymerase instead of Redtaq because the redtaq has been having some issues lately. If there is microbial DNA present then we may be able to get PCR for the CRISPR to work from it. In the meantime I am waiting for our M17 media to show up.
