"
Team:ITB Indonesia/DegMod
From 2014.igem.org
(Difference between revisions)
Mardikusumo (Talk | contribs) |
Mardikusumo (Talk | contribs) |
||
| Line 225: | Line 225: | ||
</div> | </div> | ||
| + | <br> | ||
<h1>Degradation Module</h1> | <h1>Degradation Module</h1> | ||
<p>Team ITB_Indonesia tries to make a module to degrade PET. We use LC-Cutinase fused with ompA and Lpp signal sequence. LC-Cutinase will break the ester bonds on PET structure through esterase activity of the enzyme and generate ethylene glycol and terephthalic acid as product. We plan to expose this enzyme on the surface membrane of bacteria thus making the enzyme immobile and more thermostable. On the other hand, PET which has high molecular mass can be degraded directly without need to pass through bacteria cell membrane. This molecular machine then named as whole cell biocatalyst.</p> | <p>Team ITB_Indonesia tries to make a module to degrade PET. We use LC-Cutinase fused with ompA and Lpp signal sequence. LC-Cutinase will break the ester bonds on PET structure through esterase activity of the enzyme and generate ethylene glycol and terephthalic acid as product. We plan to expose this enzyme on the surface membrane of bacteria thus making the enzyme immobile and more thermostable. On the other hand, PET which has high molecular mass can be degraded directly without need to pass through bacteria cell membrane. This molecular machine then named as whole cell biocatalyst.</p> | ||
| Line 230: | Line 231: | ||
<img src="https://static.igem.org/mediawiki/2014/3/30/Degradation-module.jpg"> | <img src="https://static.igem.org/mediawiki/2014/3/30/Degradation-module.jpg"> | ||
| - | |||
<br> | <br> | ||
Revision as of 19:05, 14 October 2014
Degradation Module
Team ITB_Indonesia tries to make a module to degrade PET. We use LC-Cutinase fused with ompA and Lpp signal sequence. LC-Cutinase will break the ester bonds on PET structure through esterase activity of the enzyme and generate ethylene glycol and terephthalic acid as product. We plan to expose this enzyme on the surface membrane of bacteria thus making the enzyme immobile and more thermostable. On the other hand, PET which has high molecular mass can be degraded directly without need to pass through bacteria cell membrane. This molecular machine then named as whole cell biocatalyst.
