Team:Marburg:Project:Notebook:April
From 2014.igem.org
(Difference between revisions)
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<!-- exp13.2 --> | <!-- exp13.2 --> | ||
<ul class="templates"> | <ul class="templates"> | ||
- | <li>Bacillus gDNA: 1:50 → 10 µL gDNA + 490 µL water</li> | + | <li><i>Bacillus</i> gDNA: 1:50 → 10 µL gDNA + 490 µL water</li> |
- | <li>pKH- | + | <li>pKH-Stp: 10 ng/µL</li> |
<li>PSG1164: 232 ng/µL → 1:20 → 10 µL plasmid + 190 µL water | <li>PSG1164: 232 ng/µL → 1:20 → 10 µL plasmid + 190 µL water | ||
</ul> | </ul> | ||
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<th scope="col"> </th> | <th scope="col"> </th> | ||
<th scope="col">1&2 plasmid PSG1146 (856 bp)</th> | <th scope="col">1&2 plasmid PSG1146 (856 bp)</th> | ||
- | <th scope="col">3&4 Bacillus gDNA (543 bp)</th> | + | <th scope="col">3&4 <i>Bacillus</i> gDNA (543 bp)</th> |
<th scope="col">5&6 plasmid KG-Stp (743 bp)</th> | <th scope="col">5&6 plasmid KG-Stp (743 bp)</th> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<th scope="row">Fragment</th> | <th scope="row">Fragment</th> | ||
- | <td>Cat- | + | <td>Cat-resistence</td> |
- | <td>amyE- | + | <td><i>amyE-gene</i></td> |
<td>GFP</td> | <td>GFP</td> | ||
</tr> | </tr> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <th scope="row">Total | + | <th scope="row">Total volume</th> |
<td>50</td> | <td>50</td> | ||
<td>50</td> | <td>50</td> | ||
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</table> | </table> | ||
<ul> | <ul> | ||
- | <li>4µL Mix + 1 µL loading dye</li> | + | <li>4 µL Mix + 1 µL loading dye</li> |
- | <li>5µL Gene ruler</li> | + | <li>5 µL Gene ruler</li> |
- | <li>5µL 2-log ladder</li> | + | <li>5 µL 2-log ladder</li> |
</ul> | </ul> | ||
</div> | </div> |
Revision as of 13:23, 14 October 2014
Notebook: April