Overview

It is known that the length of anti-sense is related to its repression efficiency (Nakashima 2006), but it is not yet found that the detail between length of anti-sense and their repression efficiency. In this study, we made many kinds of anti-sense that commonly contain anti-RBS sequence but have different length of anti-CDS sequence, and compared their repression efficiencies. The target gene was mRFP and its RBS, B0034. We made anti-senses containing B0034’s reverse sequence, each anti-sense is 30bp, 60bp, 90bp, 120bp, 150bp. 30bp anti-sense covers only B0034.

To make relationship between the length of asRNA and its repression efficiency clear, we help you make anti-sense constructs. If it were made to be clear that repression efficiency is related to anti-sense length, we would be able to make the anti-sense library that has many kinds of anti-sense which has each repression efficiency in the future.

Introduction

In repressing gene by anti-sense, it is important to choose anti-sense length. However, it is difficult to decide anti-sense length. Even if they are too long, it doesn’t repress target RNA effectively. The reason is because RNA polymerase takes a lot of time to synthesize them, and the diffusion rate of them is also made to be low. However, too short asRNA also has some problem. The short anti-sense cannot bind to the specific part of mRNA because it has too short complementary sequences of target RNA. In the industrial and academic places, it is hoped to use anti-sense that has suitable repression efficiency. For example, you can create knock down recombinant organisms easily by using strong anti-sense, and in iGEM, you can make a bio-devices which has a complicated gene network and require fine-tuned gene expression. Gene’s expression is not only ON or OFF. As stated above, each cases need each repression efficiency. Researchers currently try to change anti-sense repression efficiency by changing anti-sense’s binding sequence. However, it is found that this method is difficult.

In this study, we made many kinds of anti-sense which have different lengths, and are investigated to have different repression efficiency. We wanted to create the diversity of repression efficiency by making many kinds of anti-sense length. We expect this results will help you decide anti-sense sequences. It is our satisfaction that our study tells scientists and iGEMers that repressing by anti-sense is easy and acurate.