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| Line 90: |
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| | start: new Date(2014,7,28), | | start: new Date(2014,7,28), |
| | description: "Details: Ligation using T4 ligase and products. Incubated for 1 hr at room temperature and them left overnight in 4C. Enzymes heat-killed at 65C.<br><br>- Tom"}, | | description: "Details: Ligation using T4 ligase and products. Incubated for 1 hr at room temperature and them left overnight in 4C. Enzymes heat-killed at 65C.<br><br>- Tom"}, |
| - | { title: "Construction of pUS204, Transformaiton of ligated products into Top10 cells",
| |
| - | start: new Date(2014,8,01),
| |
| - | description: "Details: Transformation and then incubated overnight at 37 C<br><br>- Abi and Tom"},
| |
| - | { title: "Checking pUS201 construct, Re-checking the pUS201 by restriction digestion",
| |
| - | start: new Date(2014,8,04),
| |
| - | description: "Details: Restriction digestion (EcoR1, NdeI, NdeI/BamHI, NdeI/HindIII)<br><br>"},
| |
| - | { title: "construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully",
| |
| - | start: new Date(2014,8,09),
| |
| - | description: "Details: Junction PCR with iGEM1407/iGEM1408 primers and Taq<br><br>Result: Products plated for…why?<br><br>- Tom"},
| |
| - | { title: "Construction of pUS203, Checking: heat-killing and running a gel of products",
| |
| - | start: new Date(2014,8,10),
| |
| - | description: "Details: Heat killed construct at 80 C for 20 min. Ran a gel electrophoresis of products wfor 100V for 1hr.<br><br>Result: Gel failed.<br><br>Conclusion: Gibson Assembly attempted anyway, gel fail was not indication of no construct<br><br>- Tom"},
| |
| - | { title: "Construction of pUS201, Plating out Pus201 Gibson Assembly",
| |
| - | start: new Date(2014,8,10),
| |
| - | description: "Details: Plated out GA products onto Amp plates.<br><br>- Tom"},
| |
| - | { title: "Construction of pUS204, Junction PCR gel",
| |
| - | start: new Date(2014,8,10),
| |
| - | description: "Details: Gel electrophoresis with PCR products (overnight pSB1C3/aeBlue cultures, 1hr growth cultures, no plasmid colony, no template control)<br><br>Result: ??<br><br>Conclusion: ??<br><br>"},
| |
| - | { title: "Checkin pUS203, Patch plate Int1I-2 and SamR construct",
| |
| - | start: new Date(2014,8,11),
| |
| - | description: "Details: GA product cultures transferred to patch plates using 25x \"small\" colonies and 25x\"large\" colonies.<br><br>Result: Colonies to be screened with junction PCR. Why patch plate?<br><br>Conclusion: ?<br><br>- ?"},
| |
| - | { title: "Checking Pus203, Preperation of colony PCR of PUS203: of the \"small\" and \"large\" colonies on patch plate",
| |
| - | start: new Date(2014,8,12),
| |
| - | description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>"},
| |
| - | { title: "Checking Pus203, colony PCR of PUS203: of the \"small\" and \"large\" colonies on patch plate",
| |
| - | start: new Date(2014,8,15),
| |
| - | description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>Result: Expected fragment size was 473bp. No data received. ????<br><br>Conclusion: ?? Re-do?<br><br>- rokiah"},
| |
| | ] | | ] |
| | }); | | }); |
"
