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Template:Team:USyd-Australia/Calendar/Events List
From 2014.igem.org
(Difference between revisions)
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{ title: 'Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests', | { title: 'Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests', | ||
start: new Date(2014,9,7), | start: new Date(2014,9,7), | ||
| - | description: 'Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA & (A260/280) 1.62.<br><br>Conclusion: Possible protein contamination to samples<br><br>- Abi'}, | + | description: 'Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA & (A260/280) |
| + | |||
| + | 1.62.<br><br>Conclusion: Possible protein contamination to samples<br><br>- Abi'}, | ||
{ title: 'Plasmid prep of pSB6A1, Re-doing digest', | { title: 'Plasmid prep of pSB6A1, Re-doing digest', | ||
start: new Date(2014,10,7), | start: new Date(2014,10,7), | ||
| Line 44: | Line 46: | ||
{ title: 'Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ', | { title: 'Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ', | ||
start: new Date(2014,24,7), | start: new Date(2014,24,7), | ||
| - | description: 'Details: PCR Master mix with 1401/1402 primers. Used iGEM pSB6A1. Ran on regular program PCR ~2HRS<br><br>Result: Yield 1018.6ng/uL and low 260/280<br><br>Conclusion: ?<br><br>- Callum'}, | + | description: 'Details: PCR Master mix with 1401/1402 primers. Used iGEM pSB6A1. Ran on regular program PCR ~2HRS<br><br>Result: Yield 1018.6ng/uL and |
| + | |||
| + | low 260/280<br><br>Conclusion: ?<br><br>- Callum'}, | ||
{ title: 'Construction of pUS201, Checking: finish pcr, gel electrophoresis of digests', | { title: 'Construction of pUS201, Checking: finish pcr, gel electrophoresis of digests', | ||
start: new Date(2014,24,7), | start: new Date(2014,24,7), | ||
| - | description: 'Details: Gel electrophoresis (100V, 400mA, 1hr). Stained in gel red for 30min<br><br>Result: Expected fragment size of 2.6kB from pSB6A1<br><br>Conclusion: Primers amplified expected product from pSB6A1<br><br>- Callum'}, | + | description: 'Details: Gel electrophoresis (100V, 400mA, 1hr). Stained in gel red for 30min<br><br>Result: Expected fragment size of 2.6kB from |
| + | |||
| + | pSB6A1<br><br>Conclusion: Primers amplified expected product from pSB6A1<br><br>- Callum'}, | ||
{ title: 'Construction of pUS204, Restriction digest of pSB1C3', | { title: 'Construction of pUS204, Restriction digest of pSB1C3', | ||
start: new Date(2014,29,7), | start: new Date(2014,29,7), | ||
| - | description: 'Details: Restriction digest (EcoR1+Pst1)<br><br>Result: Yield 25ng/mL<br><br>Conclusion: Enough to use for gibson assembly<br><br>- Callum'}, | + | description: 'Details: Restriction digest (EcoR1+Pst1)<br><br>Result: Yield 25ng/mL<br><br>Conclusion: Enough to use for gibson assembly<br><br>- |
| + | |||
| + | Callum'}, | ||
{ title: 'Construction of pUS201, PCR of pSB1C3 using iGEM403/1404 primers ', | { title: 'Construction of pUS201, PCR of pSB1C3 using iGEM403/1404 primers ', | ||
start: new Date(2014,30,7), | start: new Date(2014,30,7), | ||
| - | description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Yield 329.4ng/uL, low 260/280<br><br>- Callum'}, | + | description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Yield 329.4ng/uL, low |
| + | |||
| + | 260/280<br><br>- Callum'}, | ||
{ title: 'Construction of pUS204, Transformation: pUS204 into top10 cells', | { title: 'Construction of pUS204, Transformation: pUS204 into top10 cells', | ||
start: new Date(2014,30,7), | start: new Date(2014,30,7), | ||
| - | description: 'Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, "far unlikely positive control worked" <br><br>- ?'}, | + | description: 'Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue |
| + | |||
| + | colonies detected<br><br>Conclusion: pUS024 did not successfully transform, "far unlikely positive control worked" <br><br>- ?'}, | ||
{ title: 'construction of pUS201, see notebook: second GA and transformation successful???', | { title: 'construction of pUS201, see notebook: second GA and transformation successful???', | ||
start: new Date(2014,0,8), | start: new Date(2014,0,8), | ||
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{ title: 'construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404', | { title: 'construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404', | ||
start: new Date(2014,3,8), | start: new Date(2014,3,8), | ||
| - | description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>- Jeanne & Callum'}, | + | description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in |
| + | |||
| + | the fridge<br><br>- Jeanne & Callum'}, | ||
{ title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ', | { title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ', | ||
start: new Date(2014,4,8), | start: new Date(2014,4,8), | ||
| - | description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- | + | description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| + | Jeanne & Callum'}, | ||
| + | { title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies', | ||
| + | start: new Date(2014,4,8), | ||
| + | description: 'Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to | ||
| + | |||
| + | use<br><br>- Jeanne & Callum'}, | ||
| + | { title: 'Checking IntI1 from SamR's construct, Restriction digest: SamR's pSB1C3+ARApBAD', | ||
| + | start: new Date(2014,7,8), | ||
| + | description: 'Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C | ||
| + | |||
| + | fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>- Rokiah'}, | ||
| + | { title: 'Checking pUS201 construct, Checking pUS201 with digests', | ||
| + | start: new Date(2014,11,8), | ||
| + | description: 'Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>- ?'}, | ||
] | ] | ||
}); | }); | ||
Revision as of 04:57, 14 October 2014
