Template:Team:USyd-Australia/Calendar/Events List
From 2014.igem.org
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{ title: 'Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests', | { title: 'Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests', | ||
start: new Date(2014,9,7), | start: new Date(2014,9,7), | ||
- | description: 'Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA | + | description: 'Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA & (A260/280) 1.62.<br><br>Conclusion: Possible protein contamination to samples<br><br>- Abi'}, |
{ title: 'Plasmid prep of pSB6A1, Re-doing digest', | { title: 'Plasmid prep of pSB6A1, Re-doing digest', | ||
start: new Date(2014,10,7), | start: new Date(2014,10,7), | ||
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{ title: 'Plasmid prep of pUS44, Re-doing digest', | { title: 'Plasmid prep of pUS44, Re-doing digest', | ||
start: new Date(2014,16,7), | start: new Date(2014,16,7), | ||
- | description: 'Details: Restriction digest. Did the protocol change?<br><br>Conclusion: Nanospec: 395.5ng/mL DNA yield<br><br>- Abi | + | description: 'Details: Restriction digest. Did the protocol change?<br><br>Conclusion: Nanospec: 395.5ng/mL DNA yield<br><br>- Abi & Tom'}, |
{ title: 'Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ', | { title: 'Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ', | ||
start: new Date(2014,24,7), | start: new Date(2014,24,7), | ||
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{ title: 'construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404', | { title: 'construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404', | ||
start: new Date(2014,3,8), | start: new Date(2014,3,8), | ||
- | description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>- Jeanne | + | description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>- Jeanne & Callum'}, |
{ title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ', | { title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ', | ||
start: new Date(2014,4,8), | start: new Date(2014,4,8), | ||
- | description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- Jeanne | + | description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- Jeanne & Callum'}, |
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{ title: 'Checking IntI1 from SamR's construct, Checking: SamR's digests on gel electrohoresis', | { title: 'Checking IntI1 from SamR's construct, Checking: SamR's digests on gel electrohoresis', | ||
start: new Date(2014,11,8), | start: new Date(2014,11,8), | ||
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{ title: 'construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully', | { title: 'construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully', | ||
start: new Date(2014,8,9), | start: new Date(2014,8,9), | ||
- | description: 'Details: Junction PCR with iGEM1407/iGEM1408 primers and Taq<br><br>Result: Products plated | + | description: 'Details: Junction PCR with iGEM1407/iGEM1408 primers and Taq<br><br>Result: Products plated for…why?<br><br>- Tom'}, |
{ title: 'Construction of pUS203, Checking: heat-killing and running a gel of products', | { title: 'Construction of pUS203, Checking: heat-killing and running a gel of products', | ||
start: new Date(2014,9,9), | start: new Date(2014,9,9), |
Revision as of 04:51, 14 October 2014