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| Line 57: |
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| | start: new Date(2014,30,7), | | start: new Date(2014,30,7), |
| | description: 'Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, "far unlikely positive control worked" <br><br>- ?'}, | | description: 'Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, "far unlikely positive control worked" <br><br>- ?'}, |
| - | { title: 'construction of pUS201, see notebook: second GA and transformation successful???',
| |
| - | start: new Date(2014,0,8),
| |
| - | description: ''},
| |
| - | { title: 'construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404',
| |
| - | start: new Date(2014,3,8),
| |
| - | description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>- Jeanne & Callum'},
| |
| - | { title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ',
| |
| - | start: new Date(2014,4,8),
| |
| - | description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- Jeanne & Callum'},
| |
| - | { title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',
| |
| - | start: new Date(2014,4,8),
| |
| - | description: 'Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>- Jeanne & Callum'},
| |
| - | { title: 'Checking IntI1 from SamR's construct, Restriction digest: SamR's pSB1C3+ARApBAD',
| |
| - | start: new Date(2014,7,8),
| |
| - | description: 'Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>- Rokiah'},
| |
| - | { title: 'Checking pUS201 construct, Checking pUS201 with digests',
| |
| - | start: new Date(2014,11,8),
| |
| - | description: 'Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>- ?'},
| |
| - | { title: 'Checking IntI1 from SamR's construct, Checking: SamR's digests on gel electrohoresis',
| |
| - | start: new Date(2014,11,8),
| |
| - | description: 'Details: Gel electrophoresis (Spe1, uncut) at 120V for 1hr. Stained in gel red for ~30min.<br><br>Result: [12.08 SamR gel]. Cut lanes travelled further than uncut, as expected.<br><br>Conclusion: NOTE:: notes at the end cannot be read<br><br>- Rokiah'},
| |
| - | { title: 'Making IntI1 for pUS203, Restriction Digest: extracting SamR's AraC-pBAD from pSB1C3',
| |
| - | start: new Date(2014,12,8),
| |
| - | description: 'Details: pSB1C3 with AraC-pBAD was incubated with *specific enzyme* at 80 C for 20 min. The enzyme was heat-killed<br><br>- andy'},
| |
| - | { title: 'Construction of pUS204, Restrction digestion of aeBlue gBlock',
| |
| - | start: new Date(2014,13,8),
| |
| - | description: 'Details: Restriction Digestion (EcoR1+Pst1)<br><br>Result: Yield: 1.6ng/uL<br><br>Conclusion: Enough yield to continue with ligation<br><br>- abi and tom'},
| |
| - | { title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',
| |
| - | start: new Date(2014,20,8),
| |
| - | description: 'Details: Plasmid prep<br><br>Result: Yield: 3680.1ng/uL. 2.03 A260/280<br><br>Conclusion: A260/280 high ratio, may have contamination<br><br>- Callum'},
| |
| - | { title: 'Construction of pUS204, Ligation of pSB1C3 and aeBlue gBlock digestion products',
| |
| - | start: new Date(2014,27,8),
| |
| - | description: 'Details: Ligation using T4 ligase and products. Incubated for 1 hr at room temperature and them left overnight in 4C. Enzymes heat-killed at 65C.<br><br>- Tom'},
| |
| | ] | | ] |
| | }); | | }); |
"
