Template:Team:USyd-Australia/Calendar/Events List

From 2014.igem.org

(Difference between revisions)
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start: new Date(2014,29,7),
start: new Date(2014,29,7),
description: 'Details: Restriction digest (EcoR1+Pst1)<br><br>Result: Yield 25ng/mL<br><br>Conclusion: Enough to use for gibson assembly<br><br>-  Callum'},
description: 'Details: Restriction digest (EcoR1+Pst1)<br><br>Result: Yield 25ng/mL<br><br>Conclusion: Enough to use for gibson assembly<br><br>-  Callum'},
 +
{ title: 'Construction of pUS201, PCR of pSB1C3 using iGEM403/1404 primers ',
 +
start: new Date(2014,30,7),
 +
description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Yield 329.4ng/uL, low 260/280<br><br>-  Callum'},
 +
{ title: 'Construction of pUS204, Transformation: pUS204 into top10 cells',
 +
start: new Date(2014,30,7),
 +
description: 'Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, "far unlikely positive control worked" <br><br>-  ?'},
 +
{ title: 'construction of pUS201, see notebook: second GA and transformation successful???',
 +
start: new Date(2014,0,8),
 +
description: ''},
 +
{ title: 'construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404',
 +
start: new Date(2014,3,8),
 +
description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>-  Jeanne & Callum'},
 +
{ title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ',
 +
start: new Date(2014,4,8),
 +
description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>-  Jeanne & Callum'},
 +
{ title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',
 +
start: new Date(2014,4,8),
 +
description: 'Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>-  Jeanne & Callum'},
 +
{ title: 'Checking IntI1 from SamR's construct, Restriction digest: SamR's pSB1C3+ARApBAD',
 +
start: new Date(2014,7,8),
 +
description: 'Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>-  Rokiah'},
 +
{ title: 'Checking pUS201 construct, Checking pUS201 with digests',
 +
start: new Date(2014,11,8),
 +
description: 'Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>-  ?'},
 +
{ title: 'Checking IntI1 from SamR's construct, Checking: SamR's digests on gel electrohoresis',
 +
start: new Date(2014,11,8),
 +
description: 'Details: Gel electrophoresis (Spe1, uncut) at 120V for 1hr. Stained in gel red for ~30min.<br><br>Result: [12.08 SamR gel]. Cut lanes travelled further than uncut, as expected.<br><br>Conclusion: NOTE:: notes at the end cannot be read<br><br>-  Rokiah'},
 +
{ title: 'Making IntI1 for pUS203, Restriction Digest: extracting SamR's AraC-pBAD from pSB1C3',
 +
start: new Date(2014,12,8),
 +
description: 'Details: pSB1C3 with AraC-pBAD was incubated with *specific enzyme* at 80 C for 20 min. The enzyme was heat-killed<br><br>-  andy'},
 +
{ title: 'Construction of pUS204, Restrction digestion of aeBlue gBlock',
 +
start: new Date(2014,13,8),
 +
description: 'Details: Restriction Digestion (EcoR1+Pst1)<br><br>Result: Yield: 1.6ng/uL<br><br>Conclusion: Enough yield to continue with ligation<br><br>-  abi and tom'},
 +
{ title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',
 +
start: new Date(2014,20,8),
 +
description: 'Details: Plasmid prep<br><br>Result: Yield: 3680.1ng/uL. 2.03 A260/280<br><br>Conclusion: A260/280 high ratio, may have contamination<br><br>-  Callum'},
 +
{ title: 'Construction of pUS204, Ligation of pSB1C3 and aeBlue gBlock digestion products',
 +
start: new Date(2014,27,8),
 +
description: 'Details: Ligation using T4 ligase and products. Incubated for 1 hr at room temperature and them left overnight in 4C. Enzymes heat-killed at 65C.<br><br>-  Tom'},
]       
]       
});
});

Revision as of 04:25, 14 October 2014

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