Team:Minnesota/Templates

From 2014.igem.org

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<h4>Attributions</h4>
<h4>Attributions</h4>
-
<br><u><b>Wet Lab:</b></u><br>
 
<br>
<br>
-
<u><b>Mercury Project design: </b></u><br>
+
Wet Lab:
-
Basem, Aunica, <br>
+
 
-
<br>
+
Mercury Project Design:  
-
<u><b>Mercury ion Testing: </b></u><br>
+
Basem, Aunica
-
Aunica, Sarah, Cassandra, Camilo, Srijay, Jennifer, Suzie<br>
+
 
-
<br>
+
Mercury Ion Testing:  
-
<u><b>Methylmercury testing:</b> </u><br>
+
Sarah, Cassandra, Camilo, Srijay, Jennifer, Suzie, Aunica
-
Nater Lab, Aunica, Nicholas, Srijay, Patrick, Suzie, Basem<br>
+
 
-
<br>
+
Methylmercury Testing:  
-
<u><b>Cadmium, Zinc, Copper project design: </b></u><br>
+
Nater Lab, Nicholas, Srijay, Patrick, Suzie, Basem, Aunica
-
Basem, Stephen, Aunica<br>
+
 
-
<br>
+
Cadmium, Zinc, Copper project design:  
-
<u><b>Cadmium, Zinc, Copper Testing: </b></u><br>
+
Basem, Stephen, Aunica
-
Aunica, Cassandra, Jessica ???<br>
+
 
-
<br>
+
Cadmium, Zinc, Copper Testing:  
-
<u><b>Kill Switch Proposal: </b></u><br>
+
Cassandra, Jessica, Aunica
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David, Sarah, Camilo, Stephen, Basem<br>
+
 
-
<br>
+
Kill Switch Biosafety Proposal:  
-
pDU1358 received from Dr. Anne O. Summers, University of Georgia<br>
+
David, Sarah, Camilo, Basem
-
pSB74 received through addgene from Keasling Lab<br>
+
 
-
<br>
+
pDU1358 received from Dr. Anne O. Summers, University of Georgia
-
<u><b>Composite parts:</b></u><br>
+
pSB74 received through addgene from Keasling Lab
-
mer operon:</b><u><br>
+
 
-
Primer design: Basem, Stephen<br>
+
Composite parts:
-
Parts cloning: Basem, Jennifer, Stephen, Valeriu<br>
+
mer operon:
-
<br>
+
Primer design: Basem, Stephen
-
<u><b>phsABC:</b></u><br>
+
Parts cloning: Basem, Jennifer, Valeriu
-
Primer design: Basem, Stephen<br>
+
 
-
Parts cloning: Basem, Stephen, Valeriu<br>
+
phsABC:
-
<br>
+
Primer design: Basem, Stephen
-
<u><b>Single parts:</b></u><br>
+
Parts cloning: Basem, Valeriu
-
<b>merR:</b></u><br>
+
 
-
Primer design: Basem, Stephen<br>
+
Single parts:
-
Parts cloning: Cassandra<br>
+
merR:
-
<u><i><b>merT:</b></i></u><br>
+
Primer design: Basem, Stephen
-
Primer design: Stephen, Basem<br>
+
Parts cloning: Cassandra
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Parts cloning: Sarah, Jennifer<br>
+
merT:
-
<u><i><b>merP:</b></i></u><br>
+
Primer design: Basem, Stephen
-
Primer design: Basem, Stephen <br>
+
Parts cloning: Sarah, Jennifer
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Parts cloning: Camilo, Logan<br>
+
merP:
-
<u><i><b>merA:</b></i></u><br>
+
Primer design: Basem, Stephen  
-
Primer design: Stephen, Basem<br>
+
Parts cloning: Camilo, Logan
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Parts cloning: Valeriu, Jessica<br>
+
merA:
-
Characterization: Cassandra, Sarah<br>
+
Primer design: Basem, Stephen
-
<i><b>merB:</b></i></u><br>
+
Parts cloning: Valeriu, Jessica
-
Primer design: Basem, Stephen<br>
+
Characterization: Cassandra, Sarah
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Parts cloning: Logan, David<br>
+
merB:
-
<br>
+
Primer design: Basem, Stephen
-
<u><b>Chassis Transformations:</b></u><br>
+
Parts cloning: Logan, David
-
<i>Pseudomonas putida:</i> Basem<br>
+
 
-
<i>Shewanella oneidensis:</i> Basem<br>
+
Chassis Transformations:
-
<i>E. coli K12:</i> Basem, David<br>
+
Pseudomonas putida: Basem
-
<br>
+
Shewanella oneidensis: Basem
-
Rhodopseudomonas</b></u><br>
+
E. coli K12: Basem, David
-
Project design: Basem, Stephen<br>
+
 
-
Parts cloning: Stephen, Basem<br>
+
 
-
<br>
+
EncapsuLab:
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<u><b>Dry lab:</b></u><br>
+
 
-
<br>
+
Encapsulation Protocol Design: Srijay, Patrick, David, Nicholas
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<u><b>EncapsuLab:</b></u><br>
+
Cell encapsulation: Nicholas, Patrick, Srijay, David, Basem, Suzie
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Protocol Design: Srijay, Patrick, David, Nicholas<br>
+
Cell Viability Testing: Patrick, David, Nicholas
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Cell encapsulation: Nicholas, Patrick, Srijay, David, Basem, Suzie<br>
+
SEM encapsulation imaging: Nicholas, UofM imaging center
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Cell Viability Testing: Patrick, David, Nicholas<br>
+
Device design: Roxana, Nicholas
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SEM encapsulation imaging: Nicholas, UofM imaging center<br>
+
Mathematical modelling: Di, Zhiyi, Patrick, David
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Device design: Roxana, Nicholas<br>
+
 
-
Mathematical modelling: Di, Zhiyi, Patrick, David<br>
+
Policies and Practices:
-
<br>
+
 
-
<u><b>Policies and Practices:</b></u><br>
+
Outreach, presentations, public perception studies
-
<br>
+
 
-
<u><b>Outreach, presentations, public perception studies</b></u><br>
+
School Curriculum design: Basem, Suzie
-
<br>
+
Science Museum Curriculum Design: Everybody
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School Curriculum design: Basem, Suzie<br>
+
Middle School Classroom outreach: Jess, Basem, Cassandra, Jennifer, Suzie
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Science Museum Curriculum Design: everybody<br>
+
Science Museum outreach: Jess, Jen, David, Sarah, Cassandra, Basem, Srijay, Di, Holly, Logan
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Middle School Classroom outreach: Jess, Basem, Cassandra, Jennifer, Suzie<br>
+
3M presentation: Suzie, Basem, Cassandra, Stephen, Jess
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Science Museum outreach: Jess, Jen, David, Sarah, Cassandra, Basem, Srijay, Di, Holly, Logan<br>
+
Cargill presentation: ???
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3M presentation: Suzie, Basem, Cassandra, Stephen, Jess<br>
+
State Fair outreach:
-
Cargill presentation: ???<br>
+
tabling & survey: Cassandra Taylor Jess Jen Basem Suzie Nicholas Stephen Roxana Di Srijay Patrick Holly Logan
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State Fair outreach:<br>
+
Survey statistics: Taylor
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tabling & survey: Cassandra Taylor Jess Jen Basem Suzie Nicholas Stephen Roxana Di <br>Srijay Patrick Holly Logan<br>
+
slideshow: Jess, David, Logan
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Survey statistics: Taylor<br>
+
giveaways: CBS, local businesses gift cards, Rob Rakow
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slideshow: Jess, David, Logan<br>
+
survey content: Everyone
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giveaways: CBS, local businesses gift cards, Rob Rakow<br>
+
State Fair game show:  Cassandra, Taylor
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survey content: everyone<br>
+
Multicultural Student Association Collaboration: Jessica
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State Fair game show presentation:  Cassandra, Taylor<br>
+
Colombia collaboration: (magnetic stirrer) Stephen
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<br>
+
 
-
Ethics :<br>
+
Ethics :
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blog: Basem, Cassandra, Logan, Jen<br>
+
Blog: Basem, Cassandra, Logan, Jen
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Documentary: Jennifer, David, Colombia iGEM team<br>
+
Documentary: Interview Questions, Content: Jennifer, David, Colombia iGEM team
-
<br>
+
Camera, Editing, Production: Connor Gleason
-
Business Plan:<br>
+
 
-
Justin, Tanner, Basem, Tamara, ?<br>
+
Business Plan:
-
<br>
+
Justin, Tanner, Basem, Tamara
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Economic Analysis: ??? + IP team at OTC<br>
+
 
-
<br>
+
Economic Analysis:  
-
Colombia collaboration: (magnetic stirrer) Stephen<br>
+
Justin, Tanner
-
Other collaborations??<br>
+
 
-
<br>
+
Intellectual Property Rights and Patenting
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<u><b>Wiki development</b></u><br>
+
Basem, Office of Technology Commercialization
-
Design: Mari, Chris, Aaron, Basem, ??<br>
+
 
-
Icons, figures development: Mari, Basem, Nicholas, ???<br>
+
Wiki development
-
Coding, CSS, javascript: Aaron, Chris, <br>
+
Design: Mari, Chris, Aaron, Tanner, Basem
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Lab notebook: Sarah Lucas<br>
+
Icons: Mari
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<br>
+
Figures development: Mari, Basem, Nicholas
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<u><b>Poster:</b></u><br>
+
Coding, CSS, javascript: Aaron, Chris,  
-
Basem, ??<br>
+
Content: Everybody
-
<br>
+
Lab notebook: Sarah Lucas
-
<u><b>Team Logo</b></u><br>
+
 
-
Nicholas<br>
+
Poster:
-
<br>
+
Basem
-
<u><b>Administrative forms, IP, safety:</b></u><br>
+
 
-
Basem<br>
+
Team Logo
-
<br>
+
Nicholas
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<u><b>Parts Submission form & shipping</b></u><br>
+
 
-
Stephen<br>
+
Administrative forms, IP, safety:
-
<br>
+
Basem
-
<u><b>Public relations and team contact</b></u><br>
+
 
-
Basem, Jessica<br>
+
Parts Submission form & shipping
-
<br>
+
Stephen
-
<u><b>Grant writing, fundraising</b></u><br>
+
 
-
Basem, Jess, David, Cassandra<br>
+
Public relations and team contact
 +
Basem, Jessica
 +
 
 +
Grant writing, fundraising
 +
Basem, Jess, David, Cassandra
 +
 
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Revision as of 22:49, 13 October 2014

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UMN iGEM 2014

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MNtallica: Cleaning Up Heavy Metal!

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Project



Mercury is a neurotoxic heavy metal with the ability to biomagnify, therefore it is a significant issue in public health and environmental studies worldwide. Its levels are continually on the rise due to copper, nickel, and gold mining activities, the industrial use of mercury catalysts, mercurial fungicides in agriculture, and the burning of fossil fuels. This has resulted in the pollution of many marine ecosystems and water reservoirs worldwide, the cleanup of which using current technology, is either not feasible or incredibly costly. This study describes the use of engineered recombinant bacteria to facilitate the biological remediation of the neurotoxin methylmercury and hazardous mercury ions from an aquatic target site into less toxic form. This synthetic microbe was incorporated in novel encapsulation technology within a cost-effective, scalable water filtering column. The employment of this device could rigorously change the practices used in mercury decontamination efforts as well as pave the way for the switch to biological rather than chemical processes. Furthermore, this technology can be applied towards bioremediation and biosensing of various other heavy metals and organic toxins in the environment.

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  • Wet Lab
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Dry Lab

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Wet Lab

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Meet The Team






Policies & Practices

Safety in the Lab

Attributions


Wet Lab: Mercury Project Design: Basem, Aunica Mercury Ion Testing: Sarah, Cassandra, Camilo, Srijay, Jennifer, Suzie, Aunica Methylmercury Testing: Nater Lab, Nicholas, Srijay, Patrick, Suzie, Basem, Aunica Cadmium, Zinc, Copper project design: Basem, Stephen, Aunica Cadmium, Zinc, Copper Testing: Cassandra, Jessica, Aunica Kill Switch Biosafety Proposal: David, Sarah, Camilo, Basem pDU1358 received from Dr. Anne O. Summers, University of Georgia pSB74 received through addgene from Keasling Lab Composite parts: mer operon: Primer design: Basem, Stephen Parts cloning: Basem, Jennifer, Valeriu phsABC: Primer design: Basem, Stephen Parts cloning: Basem, Valeriu Single parts: merR: Primer design: Basem, Stephen Parts cloning: Cassandra merT: Primer design: Basem, Stephen Parts cloning: Sarah, Jennifer merP: Primer design: Basem, Stephen Parts cloning: Camilo, Logan merA: Primer design: Basem, Stephen Parts cloning: Valeriu, Jessica Characterization: Cassandra, Sarah merB: Primer design: Basem, Stephen Parts cloning: Logan, David Chassis Transformations: Pseudomonas putida: Basem Shewanella oneidensis: Basem E. coli K12: Basem, David EncapsuLab: Encapsulation Protocol Design: Srijay, Patrick, David, Nicholas Cell encapsulation: Nicholas, Patrick, Srijay, David, Basem, Suzie Cell Viability Testing: Patrick, David, Nicholas SEM encapsulation imaging: Nicholas, UofM imaging center Device design: Roxana, Nicholas Mathematical modelling: Di, Zhiyi, Patrick, David Policies and Practices: Outreach, presentations, public perception studies School Curriculum design: Basem, Suzie Science Museum Curriculum Design: Everybody Middle School Classroom outreach: Jess, Basem, Cassandra, Jennifer, Suzie Science Museum outreach: Jess, Jen, David, Sarah, Cassandra, Basem, Srijay, Di, Holly, Logan 3M presentation: Suzie, Basem, Cassandra, Stephen, Jess Cargill presentation: ??? State Fair outreach: tabling & survey: Cassandra Taylor Jess Jen Basem Suzie Nicholas Stephen Roxana Di Srijay Patrick Holly Logan Survey statistics: Taylor slideshow: Jess, David, Logan giveaways: CBS, local businesses gift cards, Rob Rakow survey content: Everyone State Fair game show: Cassandra, Taylor Multicultural Student Association Collaboration: Jessica Colombia collaboration: (magnetic stirrer) Stephen Ethics : Blog: Basem, Cassandra, Logan, Jen Documentary: Interview Questions, Content: Jennifer, David, Colombia iGEM team Camera, Editing, Production: Connor Gleason Business Plan: Justin, Tanner, Basem, Tamara Economic Analysis: Justin, Tanner Intellectual Property Rights and Patenting Basem, Office of Technology Commercialization Wiki development Design: Mari, Chris, Aaron, Tanner, Basem Icons: Mari Figures development: Mari, Basem, Nicholas Coding, CSS, javascript: Aaron, Chris, Content: Everybody Lab notebook: Sarah Lucas Poster: Basem Team Logo Nicholas Administrative forms, IP, safety: Basem Parts Submission form & shipping Stephen Public relations and team contact Basem, Jessica Grant writing, fundraising Basem, Jess, David, Cassandra

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