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Team:Austin Texas/parts
From 2014.igem.org
(→Related Parts and Plasmids - Not Biobricked) |
(→Related Parts and Plasmids - Not Biobricked) |
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The 2014 UT Austin iGEM team felt quite strongly that we should make our ncAA reporter kit widely available as soon as possible. Unfortunately, this complex plasmid is not BioBrick legal, and after considerable consideration and e-mailing with Vinoo Selvarajah (iGEM HQ), we decided to include the relevant information here and be willing to share the kit with anyone who requests it until such time as an alternative is found. | The 2014 UT Austin iGEM team felt quite strongly that we should make our ncAA reporter kit widely available as soon as possible. Unfortunately, this complex plasmid is not BioBrick legal, and after considerable consideration and e-mailing with Vinoo Selvarajah (iGEM HQ), we decided to include the relevant information here and be willing to share the kit with anyone who requests it until such time as an alternative is found. | ||
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Thus, until such time, we have included the information for our ncAA reporter kit here. [link....?] | Thus, until such time, we have included the information for our ncAA reporter kit here. [link....?] | ||
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pFRY - Reporter plasmid with linker(AMBER). | pFRY - Reporter plasmid with linker(AMBER). | ||
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The two plasmids must be grown in parallel cultures. pFRYC controls for variance in gene expression dependent on the plasmid in the specific growth conditions and the length of the fusion protein. pFRY tests the fidelity and efficiency of the tRNA sythetase/tRNA pair. | The two plasmids must be grown in parallel cultures. pFRYC controls for variance in gene expression dependent on the plasmid in the specific growth conditions and the length of the fusion protein. pFRY tests the fidelity and efficiency of the tRNA sythetase/tRNA pair. | ||
Revision as of 21:58, 13 October 2014
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