Team:ITESM-CEM/EnzymaticKinectics
From 2014.igem.org
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<h2>Overview</h2> | <h2>Overview</h2> | ||
<p style="text-align: justify; text-justify: inter-word;">During 2014, iGEM ITESM CEM Team worked on the development of a metabolic pathway for 7-ketocholesterol degradation by enzyme therapy of human macrophages, using two hypothetic enzymes of the microorganism Rhodococcus jostii, active when the microbe grows using 7-ketocholesterol as a sole source of carbon. These enzymes (oxoacyl reductase and 7-dehydratase) were first described as potentially being used to catalyse these reactions by Mathieu (1); and were cloned and purified by iGEM ITESM CEM Team using E. coli and a diverse array of expression vectors.<br> | <p style="text-align: justify; text-justify: inter-word;">During 2014, iGEM ITESM CEM Team worked on the development of a metabolic pathway for 7-ketocholesterol degradation by enzyme therapy of human macrophages, using two hypothetic enzymes of the microorganism Rhodococcus jostii, active when the microbe grows using 7-ketocholesterol as a sole source of carbon. These enzymes (oxoacyl reductase and 7-dehydratase) were first described as potentially being used to catalyse these reactions by Mathieu (1); and were cloned and purified by iGEM ITESM CEM Team using E. coli and a diverse array of expression vectors.<br> | ||
- | However, in order to properly asses and predict the behaviour of both proteins in the cytosol of human cells, it is first necessary to numerically model their interaction and catalysis over their substrates. In order to do so, the proposed pathway must firstly be analysed.</p | + | However, in order to properly asses and predict the behaviour of both proteins in the cytosol of human cells, it is first necessary to numerically model their interaction and catalysis over their substrates. In order to do so, the proposed pathway must firstly be analysed.</p><br> |
- | <img src="https://static.igem.org/mediawiki/2014/6/6f/Ruta_metab%C3%B3lica_sin_fondo.jpg" align="left" width="600" height="423" hspace="10" BORDER=10> <br><br> <pie><p style="text-align: justify; text-justify: inter-word;"><b>Figure 1.-</b> Theoretical metabolic pathway for 7-ketocholesterol degradation in Rhodococcus jostii. Red arrows indicated the most intuitive order of reactions.</p></pie><br><br> | + | <img src="https://static.igem.org/mediawiki/2014/6/6f/Ruta_metab%C3%B3lica_sin_fondo.jpg" align="left" width="600" height="423" hspace="10" BORDER=10> <br><br> <pie><p style="text-align: justify; text-justify: inter-word;"><b>Figure 1.-</b> Theoretical metabolic pathway for 7-ketocholesterol degradation in Rhodococcus jostii. Red arrows indicated the most intuitive order of reactions.</p></pie><br><br><br><br> |
<p style="text-align: justify; text-justify: inter-word;">Figure 1 shows the general array of chemical reactions in the pathway, were red arrows indicate the direction proposed by iGEM ITESM CEM Team. Even though reactions can occur in almost any order, the most intuitive arrangement is that in which 7-ketocholesterol is converted to 7-βOH-cholesterol, which is finally transformed to regular cholesterol; these two reactions are supposed to be catalysed by oxoacyl reductase and 7-dehydratase respectively.<br><br> | <p style="text-align: justify; text-justify: inter-word;">Figure 1 shows the general array of chemical reactions in the pathway, were red arrows indicate the direction proposed by iGEM ITESM CEM Team. Even though reactions can occur in almost any order, the most intuitive arrangement is that in which 7-ketocholesterol is converted to 7-βOH-cholesterol, which is finally transformed to regular cholesterol; these two reactions are supposed to be catalysed by oxoacyl reductase and 7-dehydratase respectively.<br><br> |
Revision as of 21:07, 13 October 2014
ITESM-CEM | Enzy7-K me |
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