Team:UGA-Georgia/Notebook
From 2014.igem.org
(Difference between revisions)
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<li> Creation of general salts solution. </li> | <li> Creation of general salts solution. </li> | ||
<li> Creation of glycylglycine buffer. </li> | <li> Creation of glycylglycine buffer. </li> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5"> April </font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 1</h5> | ||
+ | <li> Learning about ribosome binding site library (RBS) and our metabolic Optflux model. </li> | ||
+ | <br> | ||
+ | <h5> Week 2</h5> | ||
+ | <li> Made agar plates</li> | ||
+ | <li> Creation of fresh wild type (WT) stocks. </li> | ||
+ | <br> | ||
+ | <h5> Week 3 </h5> | ||
+ | <li> Created diluted puromycin </li> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5"> May </font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 4 </h5> | ||
+ | <li> Inoculation and revival of frozen stocks. </li> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5"> June </font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 1</h5> | ||
+ | <li> Determined the optical density (OD) of the previously revived cultures. </li> | ||
+ | <br> | ||
+ | <h5> Week 2 </h5> | ||
+ | <li> Restriction digestion, purification, and ligation of pMEV4 and PCR products (C1 and C2).</li> | ||
+ | <li> Transformation by heat shock, plated the cells, made an agarose gel and ran the products on it. </li> | ||
+ | <li> Picked colonies from the previous day of plating. </li> | ||
+ | <li> Plasmid extraction, digestion, gel electrophoresis, and verification. </li> | ||
+ | <br> | ||
+ | <h5> Week 3</h5> | ||
+ | <li> Revival of pAW50-mCherry frozen stock. </li> | ||
+ | <li> Revival of S0001 (WT) frozen stock. </li> | ||
+ | <br> | ||
+ | <h5> Week 4 </h5> | ||
+ | <li> Prepared the revived cultures for fluorescence microscopy. </li> | ||
+ | <li> Fluorescence microscopy training. </li> | ||
+ | |||
</td> | </td> | ||
Revision as of 03:20, 13 October 2014
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Notebook | ||||
FebruaryWeek 1Week 3Week 4MarchWeek 1Week 3AprilWeek 1Week 2Week 3MayWeek 4JuneWeek 1Week 2Week 3Week 4 |