Team:USTC-China/safety

From 2014.igem.org

(Difference between revisions)
(Created page with "{{:Team:USTC-China/partials/header}} <html> <div id="main" class="row"> <div class="large-3 columns" id="side-navbar"> <div class="side-nav"> <dl class="accordion" data-acc...")
Line 2: Line 2:
<html>
<html>
<div id="main" class="row">
<div id="main" class="row">
-
<div class="large-3 columns" id="side-navbar">
+
  <div class="large-3 columns" id="side-navbar">
-
<div class="side-nav">
+
    <div class="side-nav">
-
<dl class="accordion" data-accordion>
+
      <dl class="accordion" data-accordion>
-
<dd class="accordion-navigation">
+
        <dd class="accordion-navigation">
-
<a href="#panel1">Protocols</a>
+
          <a href="#panel1">Safety</a>
-
<div id="panel1" class="content active">
+
          <div id="panel1" class="content active">
-
<ul class="side-nav">
+
          </div>
-
<li>
+
        </dd>
-
<a href="#GelElectrophoresis">
+
      </dl>
-
Gel Electrophoresis
+
    </div>
-
</a>
+
  </div>
-
</li>
+
-
<li>
+
-
<a href="#GelExtraction">
+
-
Gel Extraction
+
-
</a>
+
-
</li>
+
-
<li>
+
-
<a href="#PCRSystemPreparation">
+
-
PCR System Preparation
+
-
</a>
+
-
</li>
+
-
</ul>
+
-
</div>
+
-
</dd>
+
-
<dd class="accordion-navigation">
+
-
<a href="#panel2">
+
-
Timeline
+
-
</a>
+
-
<div id="panel2" class="content">
+
-
<ul class="side-nav">
+
-
<li>
+
-
<a href="#NULL">
+
-
NULL
+
-
</a>
+
-
</li>
+
-
</ul>
+
-
</div>
+
-
</dd>
+
-
<dd class="accordion-navigation">
+
-
<a href="#panel3">
+
-
Bibliography
+
-
</a>
+
-
<div id="panel3" class="content">
+
-
<ul class="side-nav">
+
-
</ul>
+
-
</div>
+
-
</dd>
+
-
</dl>
+
-
</div>
+
-
</div>
+
-
<div class="large-9 columns" id="content-page">
+
  <div class="large-9 columns" id="content-page">
-
<div class="title">
+
    <div class="title">
-
<h1>Protocols</h1>
+
      <h1>Safety</h1>
-
</div>
+
    </div>
-
<div class="text">
+
    <div class="text">
-
            <h2 id="safetytraininginourteam">Safety Training in our team</h2>
+
                <h2 id="safetytraininginourteam">Safety Training in our team</h2>
-
<h2 id="killswitchinecoli">Kill Switch in <em>E.coli</em></h2>
+
    <h2 id="killswitchinecoli">Kill Switch in <em>E.coli</em></h2>
-
<p>This year our safety work is based on <em>E.coli</em>, since some of our work is on light sensing and imaging system in <em>E.coli</em>. To make a wider application in the future, the biosafety of <em>E.coli</em> containing these parts should be concerned.</p>
+
    <p>This year our safety work is based on <em>E.coli</em>, since some of our work is on light sensing and imaging system in <em>E.coli</em>. To make a wider application in the future, the biosafety of <em>E.coli</em> containing these parts should be concerned.</p>
-
<p>LALF is </p>
+
    <p>LALF is </p>
-
<h3 id="antilpsfactorlalfregulatedbylaci">Anti-LPS factor(LALF) regulated by lacI</h3>
+
    <h3 id="antilpsfactorlalfregulatedbylaci">Anti-LPS factor(LALF) regulated by lacI</h3>
-
<p>We designed the part based on K541545. We found that R0010 might keep express genes below without regulation.Reason may be that Lacl expressed by E.coil is limited. So we inserted parts that express Lacl and constitutive promoter before the old one to inhibite pLac. <br />
+
    <p>We designed the part based on K541545. We found that R0010 might keep express genes below without regulation.Reason may be that Lacl expressed by E.coil is limited. So we inserted parts that express Lacl and constitutive promoter before the old one to inhibite pLac. <br />
-
<img src="/content/images/2014/Oct/QQ--20141010201844.png" alt="" /></p>
+
    <img src="/content/images/2014/Oct/QQ--20141010201844.png" alt="" /></p>
-
<h3 id="antilpsfactorlalfregulatedbyiptg">Anti-LPS factor(LALF) regulated by IPTG</h3>
+
    <h3 id="antilpsfactorlalfregulatedbyiptg">Anti-LPS factor(LALF) regulated by IPTG</h3>
-
<p>This part is a cell-kill system. Firstly,constitutive promoter could express enough Lacl that is useful for downstream. Secondly, the reporter in the middle of our part wouldn't show signal nor the cell be killed by the part behind unless IPTG or lactose added into the culture medium. No IPTG or lactose: GFP don't express ,G- won't be killed. IPTG or lactose exsisting : GFPs express ,G- will be dead. </p>
+
    <p>This part is a cell-kill system. Firstly,constitutive promoter could express enough Lacl that is useful for downstream. Secondly, the reporter in the middle of our part wouldn't show signal nor the cell be killed by the part behind unless IPTG or lactose added into the culture medium. No IPTG or lactose: GFP don't express ,G- won't be killed. IPTG or lactose exsisting : GFPs express ,G- will be dead. </p>
-
<p>G-: Gram negative bacteria <br />
+
    <p>G-: Gram negative bacteria <br />
-
<img src="/content/images/2014/Oct/QQ--20141010202045.png" alt="" /></p>
+
    <img src="/content/images/2014/Oct/QQ--20141010202045.png" alt="" /></p>
-
</div>
+
    </div>
-
</div>
+
  </div>
</div>
</div>
</html>
</html>
{{:Team:USTC-China/partials/footer}}
{{:Team:USTC-China/partials/footer}}

Revision as of 05:26, 14 October 2014

Safety

Safety Training in our team

Kill Switch in E.coli

This year our safety work is based on E.coli, since some of our work is on light sensing and imaging system in E.coli. To make a wider application in the future, the biosafety of E.coli containing these parts should be concerned.

LALF is

Anti-LPS factor(LALF) regulated by lacI

We designed the part based on K541545. We found that R0010 might keep express genes below without regulation.Reason may be that Lacl expressed by E.coil is limited. So we inserted parts that express Lacl and constitutive promoter before the old one to inhibite pLac.

Anti-LPS factor(LALF) regulated by IPTG

This part is a cell-kill system. Firstly,constitutive promoter could express enough Lacl that is useful for downstream. Secondly, the reporter in the middle of our part wouldn't show signal nor the cell be killed by the part behind unless IPTG or lactose added into the culture medium. No IPTG or lactose: GFP don't express ,G- won't be killed. IPTG or lactose exsisting : GFPs express ,G- will be dead.

G-: Gram negative bacteria

Lots of thanks to our sponsors, who help us to achieve what we have today

© 2014 USTC-iGEM Design. Distributed under BY-SA 3.0

Retrieved from "http://2014.igem.org/Team:USTC-China/safety"