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Team:Evry/Notebook/CellCharacterization/Antibiotic test/08-17-2014
From 2014.igem.org
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There was a problem with the stock of Kanamycine so we made a new one at 25mg/mL. | There was a problem with the stock of Kanamycine so we made a new one at 25mg/mL. | ||
| - | For the erythrompycine, we learn that E. Coli is not really sensitive to this antibiotic and that it's better to use it with a dilution at 1:100.<br> | + | For the erythrompycine, we learn that E. Coli is not really sensitive to this antibiotic and that it's better to use it with a dilution at 1:100.<br></p> |
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Any liquid culture can be plated and will grow up. Of course, it works better on MB medium, wich is a rich medium than on M9. | Any liquid culture can be plated and will grow up. Of course, it works better on MB medium, wich is a rich medium than on M9. | ||
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Latest revision as of 22:27, 17 October 2014
Tests of antibiotics' stocks - Results
Survivability tests - Results
| Plates | LB Agar only | LB+Cam | LB+Kan | LB+Amp | LB+Erm | LB+Tet |
| E.coli | X | 0 | X | 0 | X | 0 |
There was a problem with the stock of Kanamycine so we made a new one at 25mg/mL.
For the erythrompycine, we learn that E. Coli is not really sensitive to this antibiotic and that it's better to use it with a dilution at 1:100.
Survivability tests - Results
| liquid cultures/Plates | MB | M9 |
| MB | ++++ | ++ |
| M9 | ++ | + |
Any liquid culture can be plated and will grow up. Of course, it works better on MB medium, wich is a rich medium than on M9.
Aug 17
