Team:Toulouse/Project/Fungicides

From 2014.igem.org

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         <p class="textesimple">The main objective of SubtiTree is to ensure the <b> destruction of the pathogenic fungi </b> inside the tree. In order to achieve this goal, we built a genetic module to produce three different peptides with antifungal activities. </p> <br>
         <p class="textesimple">The main objective of SubtiTree is to ensure the <b> destruction of the pathogenic fungi </b> inside the tree. In order to achieve this goal, we built a genetic module to produce three different peptides with antifungal activities. </p> <br>
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  <p class="textesimple"> Originated from plants, these peptides have different targets to maximize the lethality on <i>C. platani</i>.  
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  <p class="textesimple">Originated from plants, these peptides have different targets to maximize the lethality on <i>C. platani</i>.  
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- <b>D4E1</b> is a synthetic peptide analog to Cecropin B AMPs (antimicrobial peptides) made of 17 amino acids which has been shown to have an antifungal activity by complexing with a sterol present in the conidia’s wall of numerous fungi. </p>
- <b>D4E1</b> is a synthetic peptide analog to Cecropin B AMPs (antimicrobial peptides) made of 17 amino acids which has been shown to have an antifungal activity by complexing with a sterol present in the conidia’s wall of numerous fungi. </p>
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<p class="title1" style="margin-top:30px;"><b>More information on this module </p></b> <br>
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<p class="title1" style="margin-top:30px;">More information on this module </p>
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We built different genetic constructions to test each fungicide separately and to test them all together on the same operon where the 3 genes coding for the antifungal peptides are placed under the control of a constitutive promoter in <i>Bacillus subtilis </i>: Pveg. </p>
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We built different genetic constructions to test each fungicide separately and to test them all together on the same operon where the 3 genes coding for the antifungal peptides are placed under the control of a constitutive promoter in <i>Bacillus subtilis</i>: Pveg. </p>
<img style="width:930px; float:left; margin: 30px 0;" src="https://static.igem.org/mediawiki/parts/d/d0/Fungicideprod.jpg">  
<img style="width:930px; float:left; margin: 30px 0;" src="https://static.igem.org/mediawiki/parts/d/d0/Fungicideprod.jpg">  
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<p  class="title1"><b>Secretion </b> </p>
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<p  class="title1">Secretion</p>
<p  class="texte">In order to export the peptides outside the bacteria, the coding sequence of D4E1 and GAFP-1 was flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.  </p> <br>
<p  class="texte">In order to export the peptides outside the bacteria, the coding sequence of D4E1 and GAFP-1 was flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.  </p> <br>
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<p  class="title1"><b>References</b> </p>
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<p  class="title1">References</p>
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Revision as of 17:26, 12 October 2014