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	<tr><td><h4>Material:</h4></td>		<tr><td><h4>Material:</h4></td>
	<tr><td>Bananas</td><td>Tap water</td>		<tr><td>Bananas</td><td>Tap water</td>

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Revision as of 08:51, 13 October 2014

Protocols / A simple method for DNA isolation

A lot of people don’t know that our food contains genetic material. Therefor we performed a DNA isolation from bananas with housekeeping articles to educate interested passerby.

Material:
Bananas	Tap water
Table salt	Dishwashing liquid as detergent
Washing agent with proteases	Hot water (60 °C)
Ice	Teaspoon
Tablespoon	Fork
Isopropyl (alcohol) (cooled)	Coffee filter
Funnel	50 mL vessels

Test execution:

• preparation of extraction solution:
1. 1 teaspoon table salt
2. 1 tablespoon dishwashing liquid
3. 10 drops washing agent
4. Fill up to 100 mL with tap water
5. Mixing by shaking
• Homogenization of a banana with the fork
• Transfer of 3 teaspoons homogenized banana into a 50mL reaction vessel with 30 mL extraction solution.
• 15 min incubation at 60 °C
• 5 min chilling on ice
• Filter the mixture into a new 50 mL vessel.
• Overlay the filtered solution with some iosporyl (alcohol)
• Wait for 10 min
• DNA is soaring into the phase of isopropyl (alcohol)