Team:TU Delft-Leiden/Project/Life science/curli/cloning/GoldenGate

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Figure 1. <b>Golden Gate Assembly of a BioBrick made up of a rhamnose promoter, <i>csgB</i> and <i>csgA</i>.</b> The BioBrick encodes a rhamnose promoter (red rectangle) and the coding sequences of <i>csgB</i> and <i>csgA</i>, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the 3 DNA pieces that were ligated into pSB1C3.  
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Figure 1. <b>Golden Gate Assembly of <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>.</b> <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a> are  made up of a rhamnose promoter, <i>csgB</i> and <i>csgA</i> or <i> csgA-HIS</i>. The BioBrick encodes a rhamnose promoter (red rectangle) and the coding sequences of <i>csgB</i> and <i>csgA</i>, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the 3 DNA pieces that were ligated into pSB1C3.  
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Revision as of 11:24, 12 October 2014

Curli Module - Cloning - Golden Gate Assembly

Figure 1. Golden Gate Assembly of BBa_K1316013 and BBa_K1316014 . BBa_K1316013 and BBa_K1316014 are made up of a rhamnose promoter, csgB and csgA or csgA-HIS. The BioBrick encodes a rhamnose promoter (red rectangle) and the coding sequences of csgB and csgA, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the 3 DNA pieces that were ligated into pSB1C3.
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