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Team:UT-Tokyo/CTCD/Content
From 2014.igem.org
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<div id = "Project-2"> | <div id = "Project-2"> | ||
<img src = "https://static.igem.org/mediawiki/2014/b/b4/SubCTC_application.png" class = "contTitle" /> | <img src = "https://static.igem.org/mediawiki/2014/b/b4/SubCTC_application.png" class = "contTitle" /> | ||
| - | <p>In our project, miRNA is used for degrading mRNA in cells that express miRNA. But if we transfect construct like [pCMV | + | <p>In our project, miRNA is used for degrading mRNA in cells that express miRNA. But if we transfect construct like [pCMV-LacI-miR A binding site] and [pCAG-LacO2-GFP][5], mRNA in cells that does not express miRNA is degraded. This system will makes many application of miRNA in iGEM possible.</p> |
<p>We focused on a tissue-specific promoter and miRNA in order to detect CTCs.As well as hematopoietic cells, many other tissues shows specific miRNA expression patterns[3]. Therefore, if by making a circuit that returns an output to a certain miRNA expression pattern, we may identify where a CTC comes from.[5]</p> | <p>We focused on a tissue-specific promoter and miRNA in order to detect CTCs.As well as hematopoietic cells, many other tissues shows specific miRNA expression patterns[3]. Therefore, if by making a circuit that returns an output to a certain miRNA expression pattern, we may identify where a CTC comes from.[5]</p> | ||
<p>In addition to the detection of CTCs, combinations of tissue-specific promoter and miRNA can be applied to many treatments. For example, suicide genes can be expressed in cancer cells in a specific tissue.[6]</p> | <p>In addition to the detection of CTCs, combinations of tissue-specific promoter and miRNA can be applied to many treatments. For example, suicide genes can be expressed in cancer cells in a specific tissue.[6]</p> | ||
Revision as of 10:00, 12 October 2014
