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| - | {{Team:Aix-Marseille/header}}
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| - | <html>
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| - | <script>
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| - | // Function to handle the movement of the sidebar in the Notebook
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| - | $(document).ready(function () {
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| - | var sidebar = $('#sidebar-notebook');
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| - | var sidebarContainer = $('#sidebar-notebook-div');
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| - | $(window).scroll(function (event) {
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| - | var ypos = $(this).scrollTop();
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| - | var scrollTop = $(window).scrollTop(),
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| - | elementOffset = sidebar.offset().top,
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| - | distance = (elementOffset - scrollTop);
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| - | var collision_top = sidebar.overlaps($('#stop_top'));
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| - | var collision_bot = sidebar.overlaps($('#stop_bot'));
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| - | if (collision_top.hits.length) { /* Collision TOP */
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| - | // To fix at the top of the .row div
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| - | sidebar.css({ top: '',
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| - | bottom: '',
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| - | position: '',
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| - | width: ''
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| - | });
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| - | sidebarContainer.css({ position:'',
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| - | bottom: '',
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| - | right: ''
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| - | });
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| - | if (distance < 141) {
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| - | // To fix at the top of the page while scrolling
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| - | sidebar.css({ top: '141px',
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| - | bottom: '',
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| - | position: 'fixed',
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| - | width: '262.5px'
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| - | });
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| - | sidebarContainer.css({ position: '',
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| - | bottom: '',
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| - | right: ''
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| - | });
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| - | }
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| - | }
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| - | else if (collision_bot.hits.length) { /* Collision BOTTOM */
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| - | // To fix at the bottom of the .row div
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| - | sidebar.css({ bottom: '',
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| - | top: '',
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| - | position: '',
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| - | width: ''
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| - | });
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| - | sidebarContainer.css({ position: 'absolute',
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| - | bottom: '0px',
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| - | right: '0px'
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| - | });
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| - | if (distance > 141) {
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| - | // To fix at the top of the page while scrolling
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| - | sidebar.css({ top: '141px',
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| - | bottom: '',
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| - | position: 'fixed',
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| - | width: '262.5px'
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| - | });
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| - | sidebarContainer.css({ position: '',
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| - | bottom: '',
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| - | right: ''
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| - | });
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| - | }
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| - | }
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| - | else {
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| - | // To fix at the top of the page while scrolling
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| - | sidebar.css({ top: '141px',
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| - | bottom: '',
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| - | position: 'fixed',
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| - | width: '262.5px'
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| - | });
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| - | sidebarContainer.css({ position: '',
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| - | bottom: '',
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| - | right: ''
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| - | });
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| - | }
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| - | });
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| - | });
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| - | </script>
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| - |
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| - |
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| - | <div class="container">
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| - | <h1 class="project-title">Notebook: RelA</h1>
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| - | <br><br>
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| - | <div id="stop_top"></div>
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| - | <div class="row" style="position:relative;" id="notebook-row">
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| - | <div class="col-md-9" id="notes"><!-- NOTES -->
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| - |
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| - | <!-- ======= WEEK 13 ======= -->
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| - | <div class="panel panel-info notes-panel">
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| - | <span class="note-tag" id="week13"></span>
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| - | <div class="panel-heading">
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| - | <h3 class="panel-title">Week 13 : 09/22/2014 ➡ 09/28/2014 <div class="pull-right"></div></h3>
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| - | </div>
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| - | <div class="panel-body">
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| - | <b>Attribution:</b> Emmanuelle Bouveret<br>
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| - | <b>Objective:</b> Test the functionality of our RelA part.<br>
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| - | <p>The pSB1K3-relA plasmid constructed by the iGEM-AMU team is expected to be able to rescue the phenotype of a MG1655∆relA mutant grown on SMG plates, a medium known to induce a strong amino acid depletion in the cells (Rudd et al.,1985) that is toxic to a ∆relA mutant.</p>
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| - | <br><br>
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| - | <ul>
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| - | <li>
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| - | <u>Day 1:</u><br>
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| - | <p>Isolated clones were restreaked on SMG plates supplemented with 50 uM Kanamycin and 0.5mM IPTG. The plates were incubated at 37°C during 48 hrs.</p>
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| - | </li>
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| - | <li>
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| - | <u>Day 2:</u><br>
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| - | <p>Electrocompetent MG1655∆<i>relA</i> (EB421 strain, Wahl <i>et al</i>. 2011) cells were electroporated with either plasmid pSB1K3-RFP as a control or pSB1K3-<i>relA</i>.</p>
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| - | </li>
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| - | <li>
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| - | <u>Day 4:</u><br>
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| - | <p>As expected, we observed that the relA construct was able to complement the MG1655∆relA strain, as seen on the following figure.</p>
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| - | <div class="media notes-media">
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| - | <img class="media-object img-rounded pull-right" src="https://static.igem.org/mediawiki/2014/6/63/AMU_Team-RelA_part.png" style="width:350px">
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| - | </div>
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| - | </li>
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| - | <li>
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| - | <p><u>SMG plate composition:</u></p>
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| - | <table class="table table-hover table-bordered notes-table">
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| - | <tbody>
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| - | <tr>
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| - | <td>M9 salts</td> <td>1X</td>
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| - | </tr>
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| - | <tr>
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| - | <td>MgSO<sub>4</sub></td> <td>1mM</td>
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| - | </tr>
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| - | <tr>
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| - | <td>CaCl<sub>2</sub></td> <td>0.1mM</td>
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| - | </tr>
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| - | <tr>
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| - | <td>VitB1</td> <td>0.5µg/ml</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Glucose</td> <td>0.2%</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Serine</td> <td>1mM</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Methionine</td> <td>1mM</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Glycine</td> <td>1mM</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Bactoagar</td> <td>15g/L</td>
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| - | </tr>
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| - | </tbody>
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| - | </table>
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| - | <p><u>References:</u></p>
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| - | <ul>
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| - | <li>Rudd KE, Bochner BR, Cashel M, Roth JR. Mutations in the spoT gene of Salmonella typhimurium: effects on his operon expression. <i>J Bacteriol</i>. ;163(2):534-42, 1985.</li>
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| - | <li>Wahl A, My L, Dumoulin R, Sturgis JN, Bouveret E.Antagonistic regulation of dgkA and plsB genes of phospholipid synthesis by multiple stress responses in <i>Escherichia coli. Mol Microbiol</i>. 80(5):1260-75, 2011.</li>
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| - | </ul>
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| - | </li>
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| - | </ul>
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| - | </div>
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| - | </div>
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| - |
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| - | </div><!-- /NOTES -->
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| - |
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| - | <!-- SIDEBAR -->
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| - | <div class="col-md-3" id="sidebar-notebook-div">
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| - | <div class="list-group" id="sidebar-notebook">
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| - |
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| - | </div>
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| - | </div>
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| - | </div>
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| - | <div id="stop_bot"></div>
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| - | </div>
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| - | </html>
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| - |
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| - | {{Team:Aix-Marseille/footer}}
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