"
Team:Brasil-SP/Project/ResponseModule
From 2014.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
<h3 align="center">Response Module</h3> | <h3 align="center">Response Module</h3> | ||
| - | <p><div align="justify">A blood sample from a healthly person, which has low levels of circulating Cystatin C, when in contact with B. subtilis cells triggers the expression of the reporter gene GFP in the biodetector, resulting in fluorescent signals. However, when a blood sample from a | + | <p><div align="justify">A blood sample from a healthly person, which has low levels of circulating Cystatin C, when in contact with B. subtilis cells triggers the expression of the reporter gene GFP in the biodetector, resulting in fluorescent signals. However, when a blood sample from a ill person, wich has high levels of circulating Cystatin C, contacts B. subtilis cells it doesn’t induce the production of GFP, generating a negative signal.</p> |
<p>The GFP was chosen because the facility to found in our laboratory, we could use another reporter gene, like YFP (yellow fluorescence protein) or RFP (red florescence protein) for exemple.</p> | <p>The GFP was chosen because the facility to found in our laboratory, we could use another reporter gene, like YFP (yellow fluorescence protein) or RFP (red florescence protein) for exemple.</p> | ||
<p>All the blood samples will be filtered through a microfluidics device, extracting just the plasma, that will be analyzed after it contacts with our modified B. subtilis.</p> | <p>All the blood samples will be filtered through a microfluidics device, extracting just the plasma, that will be analyzed after it contacts with our modified B. subtilis.</p> | ||
Revision as of 22:20, 11 October 2014
Response Module
A blood sample from a healthly person, which has low levels of circulating Cystatin C, when in contact with B. subtilis cells triggers the expression of the reporter gene GFP in the biodetector, resulting in fluorescent signals. However, when a blood sample from a ill person, wich has high levels of circulating Cystatin C, contacts B. subtilis cells it doesn’t induce the production of GFP, generating a negative signal.
The GFP was chosen because the facility to found in our laboratory, we could use another reporter gene, like YFP (yellow fluorescence protein) or RFP (red florescence protein) for exemple.
All the blood samples will be filtered through a microfluidics device, extracting just the plasma, that will be analyzed after it contacts with our modified B. subtilis.
In this device, we will store B. subtilis as spores, which are activated by rich medium (Luria-Bertani [LB]) before being used in the device. In order to induce the spore formation, the bacteria are incubated in a minimal nutrient medium (Difco Sporulation Medium [DSM]). .
