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Team:Austin Texas/interlab study
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=Devices Measured= | =Devices Measured= | ||
| + | [[File:interlabliquidcultures.jpg|290px|right]] | ||
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'''1.''' The first device is [http://parts.igem.org/Part:BBa_I20260 BBa_I20260], which is a biobrick part that contains: | '''1.''' The first device is [http://parts.igem.org/Part:BBa_I20260 BBa_I20260], which is a biobrick part that contains: | ||
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*This device is in [http://parts.igem.org/Part:pSB1C3 pSB1C3]. | *This device is in [http://parts.igem.org/Part:pSB1C3 pSB1C3]. | ||
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=Protocols= | =Protocols= | ||
| - | ==Sample Preparation== | + | ===Sample Preparation=== |
*Inoculate 10 mL of LB with frozen stocks of the three transformed clones (I20260, J23101+E0240, J23115+E0240), a cell background control (TOP10 electrocompetent cells), and an LB only control in a 50 mL Erlenmeyer flask. Grow for 16-18 hours at 37°C, shaking at 300rpm. | *Inoculate 10 mL of LB with frozen stocks of the three transformed clones (I20260, J23101+E0240, J23115+E0240), a cell background control (TOP10 electrocompetent cells), and an LB only control in a 50 mL Erlenmeyer flask. Grow for 16-18 hours at 37°C, shaking at 300rpm. | ||
*The next morning, add 10µL of each overnight culture to 10 mL of LB with three replicates of each (15 total flasks) and grow 16-18 hours at 37°C, shaking at 300rpm. | *The next morning, add 10µL of each overnight culture to 10 mL of LB with three replicates of each (15 total flasks) and grow 16-18 hours at 37°C, shaking at 300rpm. | ||
*Add 80 µL of each culture to the wells of a clear-bottomed black 96-well plate. | *Add 80 µL of each culture to the wells of a clear-bottomed black 96-well plate. | ||
| - | ==Measurement Protocol== | + | ===Measurement Protocol=== |
The samples were plated on a 96-well plate as follows: | The samples were plated on a 96-well plate as follows: | ||
*The 96-well plate was inserted into the Infinite 200 PRO Microplate Reader | *The 96-well plate was inserted into the Infinite 200 PRO Microplate Reader | ||
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*Obtain fluorescence and OD600 readings | *Obtain fluorescence and OD600 readings | ||
| + | =Microplate Reader Data= | ||
| + | <center>[[File:interlabchart.jpg|640px]]</center> | ||
=Sequencing Data= | =Sequencing Data= | ||
| - | + | =Discussion= | |
<!-- WIKI CONTENT ENDS --> | <!-- WIKI CONTENT ENDS --> | ||
Revision as of 21:25, 11 October 2014
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