Team:ETH Zurich/labblog/20140806meet

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Latest revision as of 21:25, 11 October 2014

Week 11 : PDMS chip built, First quorum sensing experiment

Wednesday, August 6th

We have a first draft of our abstract.
We have results for our first quorum sensing experiment .
We have now a proper PDMS chip for diffusion experiments.
We have a simple GFP production model for simulation of these quorum sensing experiments.
In the next weeks we will fit quorum sensing parameters thanks to the first quorum sensing experiments and our simple GFP production model, refine this Comsol diffusion model with the right quorum sensing parameters and then fit this diffusion model to diffusion experiments to find the diffusion constant.

@@ Line 7: / Line 7: @@
 * We have a first draft of our abstract.
 * We have results for our <html> <a id='insidelink' targetid='firstquorum' href="https://2014.igem.org/Team:ETH_Zurich/blog#firstquorum"> first quorum sensing experiment </a></html>.
-* We have now a proper PDMS chip for diffusion experiments :
+* We have now a proper <html><a id='insidelink' targetid='firstPDMS' href="https://2014.igem.org/Team:ETH_Zurich/blog#firstPDMS"> PDMS chip </a></html> for diffusion experiments.
-[[File:ETH Zurich Pdms1.PNG|center|500px|thumb|Our millifluidic chip for diffusion experiments]]
+*We have a <html><a id='insidelink' targetid='GFPqss' href="https://2014.igem.org/Team:ETH_Zurich/blog#GFPqss"> simple GFP production model</a></html> for simulation of these quorum sensing experiments.
-*We have a simple GFP production model for simulation of these quorum sensing experiments, by using quasi steady state aproximations (considering that binding is much faster than transcription and diffusion) :
-[[File:ETH Zurich Model1.PNG|center|600px|thumb|Equations and approximations for the model of GFP production by sensor cells used in simulation of diffusion and cross-talk experiments]]
-By using producer cells on one side of the channel and receiver cells in the other side, and putting cells in a punched cylinder in agar, we should see fluorescence appear at different times depending on the channel length (1 to 4mm) :
-[[File:ETH Zurich Model2.PNG|center|600px|thumb|Simulation of diffusion experiments : fluorescence over time for the punched cylinder design and for 4 different channel lengths]]
 * In the next weeks we will fit quorum sensing parameters thanks to the first quorum sensing experiments and our simple GFP production model, refine this Comsol diffusion model with the right quorum sensing parameters and then fit this diffusion model to diffusion experiments to find the diffusion constant.