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Team:EPF Lausanne/Parts
From 2014.igem.org
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<th>Name</th> | <th>Name</th> | ||
| - | <th>Function</th> | + | <th>Main Function</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>SmashColi</td> | <td>SmashColi</td> | ||
| - | <td> | + | <td>To be able to separate the chip in 4 different compartments and apply 4 different pressure on each row of chambers.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 20:31, 11 October 2014
PARTS
DNA parts submitted by the 2014 EPFL iGEM team
Our team submitted a total of 54 Biobricks (biobricks 51 and 52 do not exist).
| Biobrick | What it is | Function | Why do we use it? | Group |
|---|---|---|---|---|
| BBa_K1486000 | CpxR coding sequence | Transcription factor | To make most of our biobricks! | Bacteria |
| BBa_K1486001 | CpxR under arabinose promoter | Treanscription factor | Bacteria | |
| BBa_K1486002 | PAra + sfGFP CpxR [Nterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486003 | Flexible linker | Attaches two proteins together | Bacteria | |
| BBa_K1486004 | Flexible linker | Attaches two proteins together | Bacteria | |
| BBa_K1486005 | PAra + CpxR sfGFP [Cterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486006 | IFP[1] | N terminus of split IFP | Bacteria | |
| BBa_K1486007 | IFP[2] | C terminus of split IFP | Bacteria | |
| BBa_K1486008 | CxpR & Split IFP1.4 [Cterm + Cterm] | Two CpxR CDS, each C terminus attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486009 | CxpR & Split IFP1.4 [Nterm + Nterm] | Two CpxR CDS, each N terminus attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486010 | CxpR & Split IFP1.4 [Nterm + Cterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486011 | CxpR & Split IFP1.4 [Cterm + Nterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486012 | CpxR + IFP[1] | CpxR with the Nterm moiety of IFP attached at its C terminus | Intermediate & control | Bacteria |
| BBa_K1486013 | CpxR + IFP[2] | CpxR with the Cterm moiety of IFP attached at its C terminus | Intermediate & control | Bacteria |
| BBa_K1486014 | IFP[1] + CpxR | CpxR with the Nterm moiety of IFP attached at its N terminus | Intermediate & control | Bacteria |
| BBa_K1486015 | IFP[2] + CpxR | CpxR with the Cterm moiety of IFP attached at its N terminus | Intermediate & control | Bacteria |
| BBa_K1486016 | fLuc[1] | N terminus moiety of the firefly luciferase | Bacteria | |
| BBa_K1486017 | fLuc[2] | C terminus moiety of the firefly luciferase | Bacteria | |
| BBa_K1486018 | PAra + fLuc[1] + fLuc[2] | Split firefly luciferase under arabinose promoter | Control | Bacteria |
| BBa_K1486019 | rLuc[1] | C terminus moiety of the renilla luciferase | Bacteria | |
| BBa_K1486020 | rLuc[2] | N terminus moiety of the renilla luciferase | Bacteria | |
| BBa_K1486021 | PAra + rLuc[1] + rLuc[2] | Split renilla luciferase under arabinose promoter | Control | Bacteria |
| BBa_K1486022 | rLuc | Full renilla luciferase | Control | Bacteria |
| BBa_K1486023 | Yeast sfGFP | Superfolder GFP for yeast cells | Reporter | Yeast |
| BBa_K1486024 | Kan | Yeast kanamycin resistance gene | Selection marker | Yeast |
| BBa_K1486025 | ADH1 terminator | Terminator | Yeast | |
| BBa_K1486026 | Yeast sfGFP + Kan | Yeast sfGFP attached to the yeast kanamycin resistance gene | Control the expression of pbs2 | Yeast |
| BBa_K1486027 | rLuc + Kan | Renilla luciferase attached to the kanamycin resistance gene | Yeast | |
| BBa_K1486028 | Yeast sfGFP[1] | N terminal moiety of split yeast sfGFP | Yeast | |
| BBa_K1486029 | sfGFP[1] + kan | Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene | Yeast | |
| BBa_K1486030 | rLuc[1] + kan | Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene | Yeast | |
| BBa_K1486031 | Ura | CDS for Uracil (yeast selective purposes) | Confer resistance to Uracil-deprived medium | Yeast |
| BBa_K1486032 | Yeast sfGFP + Ura | Yeast sfGFP attached to the Uracil CDS | Control the expression of hog1 | Yeast |
| BBa_K1486033 | rLuc + Ura | Renilla luciferase attached to the Uracil CDS | Control the expression of hog1 | Yeast |
| BBa_K1486034 | yeast sfGFP[2] | C terminal moiety of split the yeast sfGFP | Yeast | |
| BBa_K1486035 | yeast sfGFP[2] + Ura | Cterm moiety of split yeast sfGFP attached to the Uracil CDS | Yeast | |
| BBa_K1486036 | rLuc[2] + Ura | Cterm moiety of split renilla luciferase attached to the Uracil CDS | Yeast | |
| BBa_K1486037 | linker | Attaches two proteins together | Yeast | |
| BBa_K1486038 | sfGFP[1] | N terminus moiety of split superfolder GFP | Bacteria | |
| BBa_K1486039 | sfGFP[2] | C terminus moiety of split superfolder GFP | Bacteria | |
| BBa_K1486040 | sfGFP[1] + CpxR | N terminus moiety of split sfGFP attached to CpxR | Bacteria | |
| BBa_K1486041 | sfGFP[2] + CpxR | C terminus moiety of split sfGFP attached to CpxR | Bacteria | |
| BBa_K1486042 | LZip | Monomer of leucine zipper TF | Bacteria | |
| BBa_K1486043 | LZip + split rLuc | Two Leucine Zipper monomers, each attached to a different split rLuc moiety | Control for split rLuc assays | Bacteria |
| BBa_K1486044 | mut IFP[1] | Biobrick-compatible IFP[1] | Bacteria | |
| BBa_K1486045 | mut IFP[2] | Biobrick-compatible IFP[2] | Bacteria | |
| BBa_K1486046 | CpxR promoter FW | CpxR binding-region in forward direction | Bacteria | |
| BBa_K1486047 | CpxR promoter RV | CpxR binding-region in reverse direction | Bacteria | |
| BBa_K1486048 | CpxR reporter | Calgary's CpxR reporter repaired (sequence was missing) | To see when CpxR is active | Bacteria |
| BBa_K1486049 | CpxR promoter FW + RFP | Reporter of CpxR | Test the direction of the complete CpxR promoter | Bacteria |
| BBa_K1486050 | CpxR promoter RV + RFP | Reporter of CpxR | Test the direction of the complete CpxR promoter | Bacteria |
| BBa_K1486053 | Linker | 10 amino-acid linker | Attach CheY/Z to split luciferases | Bacteria |
| BBa_K1486054 | CheY/CheZ rLuc | CheY and CheZ, each attached to a moiety of split renilla luciferase | Positive control for the split rLuc | Bacteria |
| BBa_K1486055 | CheY/CheZ fLuc | CheY and CheZ, each attached to a moiety of split firefly luciferase | Positive control for the split fLuc | Bacteria |
| BBa_K1486056 | CxpR & Split mut IFP1.4 [Cterm + Cterm] | Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP | Characterize CpxR dimerization | Bacteria |
Microfluidics parts (chips created)
Our team designed and made 4 microfluidic chips. At the beginning, we also used the MITOMI chip.
| Name | Main Function |
|---|---|
| SmashColi | To be able to separate the chip in 4 different compartments and apply 4 different pressure on each row of chambers. |
| BioPad | Final construct chip |
| SafetyColi | insert here |
| FilterColi | insert here |
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