Revision as of 16:07, 11 October 2014

August

Notebook | June | July | August | School Year

	Week Ten- August 4-10 Continued Acetylene Reduction Assay for all strains and controls. Ran light induction experiment and troubleshoot data based on results. Started design of a new positive control to more accurately find out what is wrong with our system. Had our final Worldly Wednesday at Blueberry Hill before people started leaving campus before school starts. Website rearranged formatting of the basic page. Change color scheme and background. Jeffrey left the team for the summer to go abroad.
	Week Eleven- August 11-17 Presented our preliminary results to Pakrasi Group and all of the advisors. Ben started and tested new positive control and found that it did not fluoresce as expected and suspect a faulty RBS. Started design of primers to modify the RBS of the system.
	Week Twelve- August 25 - End School year started. Rebstock group continued to use acetylene reduction assay to test nitrogen fixation activity of JM109 and WM1788 under known optimal conditions. Meanwhile Rebstock group started to plan specific protocol and design for BioBricking the nif plasmid. Ben starting cloning project to test whether switching the rbs to a comparable one would fix the EYFP expression in the light plasmids. He sent the clones in for sequencing. Ben also worked on the title and abstract for the iGEM September 1st deadline.

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 <td style="vertical-align: top; text-align: center;"><span style="font-weight: bold;">Week Twelve- August 25 - End</span><br><div style="text-align: left;">
-School year started. <br>Continued cloning projects and troubleshooting.<br>Ben starting cloning for modified RBS positive control to test disfunctional reporter protein.<br>Started biobricking.<br>Caroline continued work with the positive control and started biobricking the <i>nif</i> cluster. <br> Richard continued Acetylene Reduction Assays <br>Ben confirmed that Ptet was the issue and swapped out tet promoters to a functional one. Cheryl helped Ben to swap out reporter genes into the light plasmids, and Ben successfully sent in two parts for biobricking. <br> Website started mass compiling information and formatting. Discovered Kompozer to format all the text. Worked on protocol page and notebook pages, waiting for data and graphs to fill pages.
+School year started. <br>Rebstock group continued to use acetylene reduction assay to test nitrogen fixation activity of JM109 and WM1788 under known optimal conditions.<br>
+Meanwhile Rebstock group started to plan specific protocol and design for BioBricking the <i>nif</i> plasmid.<br>
+Ben starting cloning project to test whether switching the rbs to a comparable one would fix the EYFP expression in the light plasmids. He sent the clones in for sequencing.</i>
+Ben also worked on the title and abstract for the iGEM September 1st deadline.
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Team:WashU StLouis/Notebook/August

From 2014.igem.org

Revision as of 16:07, 11 October 2014

August

Notebook | June | July | August | School Year

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