Team:INSA-Lyon/molecular

From 2014.igem.org

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<h5 align="left">A Molecular model to understand the behaviour of the invention</h5>
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                        <p><div align = "justify"> One of the main goals of our modeling work this year was to understand the <b>structure</b> of the curlin subunit protein, <b>CsgA</b> and it's behavior when engineered with a tag constituted of either six histidines (that we will call <b>His1-tag</b> from now on) or twice that motif (<b>His2-tag</b>), since such a peptide  is known for its nickel chelation properties.<br\>
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                          <h6 align="left">Depuis Lundi, l'harcèlement</h6>
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We then discussed over our results with the wetlab members to define a way to confirm the accuracy of our model, and so we were able to assess that, in accordance with litterature, the <b>best position</b> for the tag was by the <b>C-terminus</b> of the protein. We also determined that the His-tag was likely to take <b>floating conformation</b> instead of folding itself around CsgA. </div></p>
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                            <p class="colonne1">Lundi matin, l'empereur sa femme et le petit prince sont venus chez moi pour me sérer la pince
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Comme j'étais parti, le petit prince a dit, puisque c'est ainsi nous reviendrons Mardi.</p>
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<p class="colonne1">Mardi matin, l'empereur sa femme et le petit prince sont venus chez moi pour me serrer la pince
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<h6 align="left">Methods</h6>
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Comme j'étais parti, le petit prince a dit, puisque c'est ainsi nous reviendrons Mercredi.</p>
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<p class="colonne1">Mercerdi matin, l'empereur sa femme et le petit prince sont venus chez moi pour me serrer la pince
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<h5 align="left">CsgA Engineering</h5>
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Comme j'étais parti, le petit prince a dit, puisque c'est ainsi nous reviendrons Jeudi.</p>
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<p class="colonne1">Jeudi matin, l'empereur sa femme et le petit prince sont venus chez moi pour me serrer la pince
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Comme j'étais parti, le petit prince a dit, puisque c'est ainsi nous reviendrons Vendredi.</p>
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<p class="colonne1">Vendredi matin, l'empereur sa femme et le petit prince sont venus chez moi pour me serrer la pince
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Comme j'étais parti, le petit prince a dit, puisque c'est ainsi nous reviendrons Samedi.</p>
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<p class="colonne1">Samedi matin, l'empereur sa femme et le petit prince sont venus chez moi pour me serrer la pince
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Comme j'étais parti, le petit prince a dit, puisque c'est ainsi nous reviendrons Dimanche.</p>
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Revision as of 13:44, 11 October 2014

Curly'on - IGEM 2014 INSA-LYON

IGEM

MOLECULAR MODELISATION

One of the main goals of our modeling work this year was to understand the structure of the curlin subunit protein, CsgA and it's behavior when engineered with a tag constituted of either six histidines (that we will call His1-tag from now on) or twice that motif (His2-tag), since such a peptide is known for its nickel chelation properties. We then discussed over our results with the wetlab members to define a way to confirm the accuracy of our model, and so we were able to assess that, in accordance with litterature, the best position for the tag was by the C-terminus of the protein. We also determined that the His-tag was likely to take floating conformation instead of folding itself around CsgA.

Methods
CsgA Engineering

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