|
|
Line 91: |
Line 91: |
| <li>Chemical safety: working with different chemicals and how to dispose of them | | <li>Chemical safety: working with different chemicals and how to dispose of them |
| </li> | | </li> |
- | <li>GMO safety: putting all waste that has been in contact with bacteria (living or dead) to bacterial waste, aseptical working methods | + | <li>GMO safety: putting all waste that has been in contact with bacteria (living or dead) to bacterial waste, aseptic working methods |
| </li> | | </li> |
| </ul> | | </ul> |
Line 210: |
Line 210: |
| <p>Perfecting everything and admiring our wiki which is totally done at this point. All's well, we can just relax before the Jamboree.</p> | | <p>Perfecting everything and admiring our wiki which is totally done at this point. All's well, we can just relax before the Jamboree.</p> |
| </div> | | </div> |
| + | </div> |
| + | </li> |
| + | |
| + | <li class="entry timeline-inverted"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>Our New Biobricks Were Sequenced</h4> |
| + | <p><small class="text-muted"> 9.10.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>We didn’t have time to sequence our new Biobricks before sending them to iGEM HQ but we wanted to sequence them anyway, just in case. The sequences were confirmed to be ok and we were really happy about this since we did have some doubts about synthesizing parts with PCR on our own.</p> |
| + | </div> |
| + | </div> |
| + | |
| + | <li class="entry timeline-inverted"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>Our Biobricks Started Their Journey to Boston</h4> |
| + | <p><small class="text-muted"> 6.10.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>Finally we got our own Biobricks ready to be sent to iGEM HQ. We put them in a box and wished them a safe journey.</p> |
| + | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/b/b3/AaltoHelsinki_shipment.JPG" class="img-responsive"> |
| </div> | | </div> |
| </li> | | </li> |
Line 224: |
Line 248: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Testing the first DNA constructs.</p> | + | <p>Testing the first DNA constructs. The results seemed promising.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 236: |
Line 260: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We plan to go to an escape the room thing, but when all the suitable times are booked, Minttu takes us bowling instead.</p> | + | <p>We planned to go to an escape the room thing, but when all the suitable times are booked, Minttu took us bowling instead.</p> |
| + | </div> |
| + | </div> |
| + | </li> |
| + | |
| + | <li class="entry timeline-inverted"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>The LEDrig Is Ready!</h4> |
| + | <p><small class="text-muted"> 22.8.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>It’s like our bacteria got their own personalized disco!</p> |
| + | <p>KUVA TULOSSA!</p> |
| + | </div> |
| + | </div> |
| + | |
| + | <li class="entry timeline-inverted"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>A Microscope That Can Almost Make You a Cup of Coffee</h4> |
| + | <p><small class="text-muted"> 21.8.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>We got to try a super cool programmable microscope (worth 120 000€!). We wanted to see if our cells produced any GFP but unfortunately it turned out that all our cells were already dead so there was no GFP to be detected.</p> |
| </div> | | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/3/3f/AaltoHelsinki_supermicroscope.jpg" class="img-responsive"> |
| </div> | | </div> |
| </li> | | </li> |
Line 248: |
Line 297: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p> We go visit the observatorium during the Night of the Arts event in Helsinki. There's also massive soap bubbles and a man with a whole band setup in a baby carriage.</p> | + | <p> We went to visit the observatorium during the Night of the Arts event in Helsinki. There were also massive soap bubbles and a man with a whole band setup in a baby carriage.</p> |
| | | |
- | <p> The next day we go back to playing pinball and Crash Team Racing because it was so much fun last week.</p> | + | <p> The next day we went back to playing pinball and Crash Team Racing because it was so much fun last week.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 258: |
Line 307: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>Testing Starts!</h4> | + | <h4>Testing Started!</h4> |
| <p><small class="text-muted"> 19.8.2014</small></p> | | <p><small class="text-muted"> 19.8.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We build the first part of the prototype and start testing!</p> | + | <p>We built the first part of the prototype and started testing!</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 270: |
Line 319: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>Our Synthetized Genes Arrive!</h4> | + | <h4>Our Synthetized Genes Arrived!</h4> |
| <p><small class="text-muted"> 18.8.2014</small></p> | | <p><small class="text-muted"> 18.8.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>After a long while, our genes finally arrive. Now we can take them and other parts we've assembled and the whole genetically engineered machine together.</p> | + | <p>After a long while, our genes finally arrived. Now we could take them and other parts we had assembled and put the whole genetically engineered machine together.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 286: |
Line 335: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We meet the event manager and event organizer of Heureka and come up with a synthetic biology weekend workshop we could organize in Spring.</p> | + | <p>We met the event manager and event organizer of Heureka and came up with a synthetic biology weekend workshop we could organize in Spring.</p> |
| | | |
- | <p>They were very excited about us and everything we had to show and tell. They said that this is a perfect match from them and all the simulations, games, pictures fit perfectly into the workshop.</p> | + | <p>They were very excited about us and everything we had to show and tell. They said that this is a perfect match for them and all the simulations, games, pictures fit perfectly into the workshop.</p> |
| | | |
| <p>We hope to inspire young people to study synthetic biology in the future and let the older people know it exists. This will also be a perfect opportunity for the next year's iGEM Team to get involved and organize something together.</p> | | <p>We hope to inspire young people to study synthetic biology in the future and let the older people know it exists. This will also be a perfect opportunity for the next year's iGEM Team to get involved and organize something together.</p> |
Line 302: |
Line 351: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Lassi takes us to play pinball. It's surprisingly fun. We play a ton of Crash Team Racing too.</p> | + | <p>Lassi took us to play pinball. It was surprisingly fun. We played a ton of Crash Team Racing too.</p> |
| </div> | | </div> |
| + | </div> |
| + | </li> |
| + | |
| + | <li class="entry timeline-inverted"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>Early Version of the LEDrig is ready!</h4> |
| + | <p><small class="text-muted"> 14.8.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>Pietu had been busy making the LEDrig inspired by Tabor Lab. This is what the early version looked like. The LEDrig would be the tool to control our light sensitive bacteria.</p> |
| + | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/7/73/AaltoHelsinki_earlyLEDrig.jpg" class="img-responsive"> |
| </div> | | </div> |
| </li> | | </li> |
Line 310: |
Line 372: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>HYBER Takes Interest In Us!</h4> | + | <h4>HYBER Took Interest In Us!</h4> |
| <p><small class="text-muted"> 8.8.2014</small></p> | | <p><small class="text-muted"> 8.8.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We meet with The Academy of Finland's Center of Excellence HYBER 2014-2019. They want us to talk in their conference and support us. </p> | + | <p>We met with The Academy of Finland's Center of Excellence HYBER 2014-2019. They wanted us to talk in their conference and support us. </p> |
| | | |
- | <p>In the evening Oskari takes us to play laser tag! He is way better at it than anyone else.</p> | + | <p>In the evening Oskari took us to play laser tag! He was way better at it than anyone else.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 324: |
Line 386: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>The First Sequencing Results Arrive!</h4> | + | <h4>The First Sequencing Results Arrived!</h4> |
| <p><small class="text-muted"> 5.8.2014</small></p> | | <p><small class="text-muted"> 5.8.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We get our first sequencing results that are in confusing .ab1-files. Then it turns out that Geneious makes analysing them incredibly easy. Most of the sequences look good, some not so much.</p> | + | <p>We got our first sequencing results that were in confusing .ab1-files. Then it turned out that Geneious makes analysing them incredibly easy. Most of the sequences looked good, some not so much.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 340: |
Line 402: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Instead of staying in our HQ and eating in the cafeteria next to it, Laura takes us to eat fancy sushi. It's delicious and fun.</p> | + | <p>Instead of staying in our HQ and eating in the cafeteria next to it, Laura took us to eat fancy sushi. It was delicious and fun.</p> |
| | | |
| <p>SUSHIKUVA?</p> | | <p>SUSHIKUVA?</p> |
Line 356: |
Line 418: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We build the sequences to synthetize from scratch. They include the lambda repressor <em>O</em><sub>R</sub> sites that we designed. We find out that the synthetisizing costs the same for 0-1000 nucleotides, so we include a few surrounding BioBricks to save on lab time. (The <em>O</em><sub>R</sub> sites are fairly small.)</p> | + | <p>We built the sequences to synthetize from scratch. They included the lambda repressor <em>O</em><sub>R</sub> sites that we designed. We found out that the synthetisizing costs the same for 0-1000 nucleotides, so we include a few surrounding BioBricks to save on lab time. (The <em>O</em><sub>R</sub> sites are fairly small.)</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 364: |
Line 426: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>The First Ligations Are Sent to Be Sequenced</h4> | + | <h4>The First Ligations Were Sent to Be Sequenced</h4> |
| <p><small class="text-muted"> 28.7.2014</small></p> | | <p><small class="text-muted"> 28.7.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We have a number of ligations ready for assembling and it turns out there's a sequencing place like 5 kilometers away.</p> | + | <p>We had a number of ligations ready for assembling and it turned out there was a sequencing lab like 5 kilometers away.</p> |
- | <p>We seal 16 samples into tubes and send them off. We are left eagerly waiting to see if the work this far has succeeded.</p> | + | <p>We sealed 16 samples into tubes and sent them off. We were left eagerly awaiting to see if the work this far had succeeded.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 381: |
Line 443: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p> We meet Tania, an ex-iGEMer from the <a href=""> one 2012 Amsterdam team.</a> It's a great meeting and we finally get some validation that we're doing some things right.</p> | + | <p> We meet Tania, an ex-iGEMer from the <a href=""> one 2012 Amsterdam team.</a> It was a great meeting and we finally got some validation that we're doing some things right.</p> |
| | | |
- | <p>In the evening Martina takes us geocaching! It is amazing what can be hidden in completely plain sight.</p> | + | <p>In the evening Martina took us geocaching! It is amazing what can be hidden in completely plain sight.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 395: |
Line 457: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We present our idea to hundreds of people and hold a booth for 6 hours.</p> | + | <p>We presented our idea to hundreds of people and held a booth for 6 hours.</p> |
| <img src="https://static.igem.org/mediawiki/2014/0/06/Aalto_Helsinki_Demoday.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2014/0/06/Aalto_Helsinki_Demoday.jpg" class="img-responsive"> |
| </div> | | </div> |
Line 408: |
Line 470: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>In the evening Mikko takes us to watch The Skin I Live In, Scott Pilgrim vs. The World and The Grand Budapest Hotel.</p> | + | <p>In the evening Mikko took us to watch The Skin I Live In, Scott Pilgrim vs. The World and The Grand Budapest Hotel.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 416: |
Line 478: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>The Gene Circuit Is Finished</h4> | + | <h4>The Gene Circuit Was Finished</h4> |
| <p><small class="text-muted"> 14.7.2014</small></p> | | <p><small class="text-muted"> 14.7.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We have the lambda repressor figured out and integrated into our system. We start planning for the parts to be synthesized.</p> | + | <p>We had the lambda repressor figured out and integrated into our system. We started planning for the parts to be synthesized.</p> |
- | | + | |
| <img src="https://static.igem.org/mediawiki/2014/2/2d/Aalto_Helsinki_Genecircus.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2014/2/2d/Aalto_Helsinki_Genecircus.jpg" class="img-responsive"> |
| </div> | | </div> |
Line 434: |
Line 495: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Summer of Startups takes us to visit the Startup people of Tallinn. We decide this is a practice trip for Boston, so we planned to have as much fun as possible. It was a great success and an unforgettable experience.</p> | + | <p>Summer of Startups took us to visit the Startup people of Tallinn. We decided this was a practice trip for Boston, so we planned to have as much fun as possible. It was a great success and an unforgettable experience.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2014/f/f9/Aalto_Helsinki_Tallinna.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2014/f/f9/Aalto_Helsinki_Tallinna.jpg" class="img-responsive"> |
Line 447: |
Line 508: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p> We find out that the lambda repressor system would be perfect for our three-way switch idea. We figure out how it works and how to include it in our gene circuit.</p> | + | <p> We found out that the lambda repressor system would be perfect for our three-way switch idea. We figured out how it worked and how to include it in our gene circuit.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 455: |
Line 516: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>The "Fun Master" System Begins</h4> | + | <h4>The "Fun Master" System Began</h4> |
| <p><small class="text-muted"> 4.7.2014</small></p> | | <p><small class="text-muted"> 4.7.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We decided that we should do something fun every week as a group that has nothing to do with iGEM. Every team member has their own week to plan an activity and pull the team to the mandatory weekly having-fun moment.</p> | + | <p>We decided that as a group we should do something fun every week that has nothing to do with iGEM. Every team member had their own week to plan an activity and pull the team to the mandatory weekly having-fun moment.</p> |
| | | |
| <p>So, Otto surprised us with a fruit juicer and a ton of fresh fruit. We spent the early morning happily drinking smoothies while working intensely on our project.</p> | | <p>So, Otto surprised us with a fruit juicer and a ton of fresh fruit. We spent the early morning happily drinking smoothies while working intensely on our project.</p> |
Line 486: |
Line 547: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>Brian Is Born</h4> | + | <h4>Brian Was Born</h4> |
| <p><small class="text-muted">1.7.2014, 2.7.2014</small></p> | | <p><small class="text-muted">1.7.2014, 2.7.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We get the idea of explaining synthetic biology as turning bacteria into superheroes from a meeting with ???. We start working on a new pitch based on that.</p> | + | <p>We got the idea of explaining synthetic biology as turning bacteria into superheroes from a meeting with ???. We started working on a new pitch based on that.</p> |
| | | |
- | <p>Pietu finishes the pitch the next day and presents it at Summer of Startups. It seems to go over very well. People finally understand what we are doing.</p> | + | <p>Pietu finished the pitch the next day and presented it at Summer of Startups. It seemed to go over very well. People finally understood what we were doing.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 506: |
Line 567: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Our BioBrick Seeker has been mentioned on the official help page for a while. We only noticed it now.</p> | + | <p>Our BioBrick Seeker had been mentioned on the official help page for a while. We only noticed it now.</p> |
| | | |
| <p>We also wrote a small advertisement text for the Community page and got the Seekers featured there.</p> | | <p>We also wrote a small advertisement text for the Community page and got the Seekers featured there.</p> |
Line 518: |
Line 579: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>The First BioBrick Ligation Combo Works</h4> | + | <h4>The First BioBrick Ligation Combo Worked</h4> |
| <p><small class="text-muted"> 24.6.2014</small></p> | | <p><small class="text-muted"> 24.6.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
| <p>We even checked it with a gel.</p> | | <p>We even checked it with a gel.</p> |
- | <p>KUVA??</p>
| |
| </div> | | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/c/cd/AaltoHelsinki_ligationgel.jpg" class="img-responsive"> |
| + | </div> |
| + | </li> |
| + | |
| + | <li class="entry timeline-inverted"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>We Had Visitors in the Lab</h4> |
| + | <p><small class="text-muted"> 23.6.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>The Summer of Startups people came to visit our lab and see what we’re actually doing.</p> |
| + | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/0/0a/AaltoHelsinki_SoSLab.JPG" class="img-responsive"> |
| + | </div> |
| + | </li> |
| + | |
| + | <li class="entry timeline-inverted"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>Backbones</h4> |
| + | <p><small class="text-muted"> 13.6.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>We found out that the Registry provides BBa_J04450 parts in different backbones, which could be used as destination plasmids. The red fluorescent protein insert makes it easier to screen out the background.</p> |
| + | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/7/7f/AaltoHelsinki_RFPbackbone.JPG" class="img-responsive"> |
| + | </div> |
| + | </li> |
| + | |
| + | <li class="entry timeline-inverted"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>Gradient PCR</h4> |
| + | <p><small class="text-muted"> 12.6.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>We kept getting weird results from PCR so we tried to solve the issue by doing a gradient PCR with different annealing temperatures. Seemed like 55 °C would be the answer to our problems.</p> |
| + | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/2/27/AaltoHelsinki_gradientPCR.jpg" class="img-responsive"> |
| + | </div> |
| + | </li> |
| + | |
| + | <li class="entry timeline-inverted"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>First Colony PCR - no succesful ligations</h4> |
| + | <p><small class="text-muted"> 11.6.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>We made our first colony PCR to test if the ligations seemed right. Unfortunately they didn’t. We decided to make linearized plasmid backbones to increase the efficiency.</p> |
| + | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/3/31/AaltoHelsinki_firstPCR.jpg" class="img-responsive"> |
| </div> | | </div> |
| </li> | | </li> |
Line 535: |
Line 648: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We're trying to advertise ourselves and get some kind of financial help and support for this project. It feels really difficult and time consuming. We also took some new pictures, a new group photo, for example.</p> | + | <p>We were trying to advertise ourselves and get some kind of financial help and support for this project. It felt really difficult and time consuming. We also took some new pictures, a new group photo, for example.</p> |
| <p><img src="http://aaltohelsinki.com/img/igem_aalto_helsinki_2014.jpg" class="img-responsive"></img></p> | | <p><img src="http://aaltohelsinki.com/img/igem_aalto_helsinki_2014.jpg" class="img-responsive"></img></p> |
| </div> | | </div> |
Line 545: |
Line 658: |
| <div class="tl-heading"> | | <div class="tl-heading"> |
| <h4>Trouble in the Lab</h4> | | <h4>Trouble in the Lab</h4> |
- | <p><small class="text-muted"> 8.6.2014</small></p> | + | <p><small class="text-muted"> 9.6.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>The backbones don't seem to be working right and the negative control keeps growing. Not good.</p> | + | <p>The backbones did't seem to be working right and the negative control kept growing. Not good.</p> |
| + | <p>The reason seemed to be that we hadn’t gel purified the backbone and it kept religating with itself.</p> |
| </div> | | </div> |
| + | </div> |
| + | </li> |
| + | |
| + | <li class="entry timeline-inverted"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>The Ligation Calculator</h4> |
| + | <p><small class="text-muted"> 5.6.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>We made a ligation calculator to help us estimate the amounts of different plasmids to be digested. The calculator calculates how many microliters of miniprepped plasmid is needed to be digested in order to pipet 4 ul of each digestion mix to the ligation mix. Insert-plasmid ratio as well as vector mass fields could be changed if needed. Taking these two variables into account, the appropriate molar amount and then the mass amount of inserts are calculated using the information of their lengths. This tiny little excel tool really saved time in the lab during the summer.</p> |
| + | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/4/4d/AaltoHelsinki_ligationcalculator.png" class="img-responsive"> |
| </div> | | </div> |
| </li> | | </li> |
Line 556: |
Line 683: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>BioBrick Seeker spreading</h4> | + | <h4>BioBrick Seeker Spreading</h4> |
| <p><small class="text-muted"> 2.6.2014</small></p> | | <p><small class="text-muted"> 2.6.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We've received tons of mentions on Twitter. People really seem to like the tool.</p> | + | <p>We've received tons of mentions on Twitter. People really seemed to like the tool.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 590: |
Line 717: |
| </div> | | </div> |
| </div> | | </div> |
| + | </li> |
| | | |
| <li class="entry timeline-inverted"> | | <li class="entry timeline-inverted"> |
Line 595: |
Line 723: |
| <div class="tl-heading"> | | <div class="tl-heading"> |
| <h4>The First BioBrick Transformation in Finland</h4> | | <h4>The First BioBrick Transformation in Finland</h4> |
- | <p><small class="text-muted">26.5.2014</small></p> | + | <p><small class="text-muted"> 27.5.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Cooool. Here it is!</p> | + | <p>Cooool. There it was!</p> |
| <img src="https://static.igem.org/mediawiki/2014/3/30/Aalto_Helsinki_First_Transformation.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2014/3/30/Aalto_Helsinki_First_Transformation.jpg" class="img-responsive"> |
| </div> | | </div> |
| + | </div> |
| + | </li> |
| + | |
| + | <li class="entry timeline-inverted"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>The First Biobrick Out of the Plate</h4> |
| + | <p><small class="text-muted"> 26.5.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>We got our hands on the Biobrick plates and took the first piece out. This is where it all started.</p> |
| + | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/2/2f/Aalto_Helsinki_Distribkit.jpg" class="img-responsive"> |
| </div> | | </div> |
| </li> | | </li> |
Line 607: |
Line 748: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>Website Is Done</h4> | + | <h4>Website Was Done</h4> |
| <p><small class="text-muted"> 23.5.2014</small></p> | | <p><small class="text-muted"> 23.5.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>This time it does actually look cool! The actual website might still be available on <a href="http://aaltohelsinki.com/index_en.html">http://aaltohelsinki.com/index_en.html</a>: check it out!</p> | + | <p>This time it did actually look cool! The actual website might still be available on <a href="http://aaltohelsinki.com/index_en.html">http://aaltohelsinki.com/index_en.html</a>: check it out!</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 619: |
Line 760: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>Lab Kicks Off!</h4> | + | <h4>Lab Kicked Off!</h4> |
| <p><small class="text-muted"> 20.5.2014</small></p> | | <p><small class="text-muted"> 20.5.2014</small></p> |
| </div> | | </div> |
Line 637: |
Line 778: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We get one desk from the laboratory. and there's still someone else's stuff on it, but it's ours and we are going to do amazing things there.</p> | + | <p>We got one desk from the laboratory. and there was still someone else's stuff on it, but it was ours and we were going to do amazing things there.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 645: |
Line 786: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>The iGEM 2014 Distribution Kit Arrives</h4> | + | <h4>The iGEM 2014 Distribution Kit Arrived</h4> |
| <p><small class="text-muted"> 9.5.2014</small></p> | | <p><small class="text-muted"> 9.5.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>The competition starts to feel real.</p> | + | <p>We got the eagerly awaited box from iGEM and the competition started to feel real. In the box we found plates full of Biobricks and other cool stuff like these pins!</p> |
| </div> | | </div> |
- | <img src="https://static.igem.org/mediawiki/2014/2/2f/Aalto_Helsinki_Distribkit.jpg" class="img-responsive"> | + | <img src="https://static.igem.org/mediawiki/2014/0/05/AaltoHelsinki_pinssi.jpg" class="img-responsive"> |
| </div> | | </div> |
| </li> | | </li> |
Line 664: |
Line 805: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We meet a few times to go over what we will say to the Summer of Startups panel, so that they will accept us to the program. This culminates on the 28th, when we have the interview. Our team leader tries to attend through Skype, but the panel doesn't like it. We manage to pull through anyway, and the panel is very excited about us at the end.</p> | + | <p>We met a few times to go over what we would say to the Summer of Startups panel, so that they would accept us to the program. This culminated on the 28th, when we had the interview. Our team leader tried to attend through Skype, but the panel didn't like it. We managed to pull through anyway, and the panel was very excited about us at the end.</p> |
| <p>They accepted us in the program.</p> | | <p>They accepted us in the program.</p> |
| </div> | | </div> |
Line 689: |
Line 830: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We presented our initial ideas to our advisor, Markus, in preparation for the expert meet. We discussed what direction to go and what kind of idea to pick.</p> | + | <p>We presented our initial ideas to our advisor, Markus, in preparation for the expert meeting. We discussed what direction to go and what kind of an idea to pick.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 752: |
Line 893: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Oskari is in the first job interview in his life, as the interviewer. He holds 19 more interviews during the week. Nine people are chosen to be in the final team.</p> | + | <p>Oskari was in the first job interview in his life, as the interviewer. He hoeld 19 more interviews during the week. Nine people were chosen to be in the final team.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 766: |
Line 907: |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>It seems like the universities are into the idea.</p> | + | <p>It seemed like the universities were into the idea.</p> |
| </div> | | </div> |
| </div> | | </div> |
Line 774: |
Line 915: |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>The Idea of a Finnish iGEM Team Forms</h4> | + | <h4>The Idea of a Finnish iGEM Team Was Formed</h4> |
| <p><small class="text-muted"></i>28.8.2013</small></p> | | <p><small class="text-muted"></i>28.8.2013</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>First e-mails about the idea are sent.</p> | + | <p>First e-mails about the idea were sent.</p> |
| </div> | | </div> |
| </div> | | </div> |