Interlab Study Project

Devices Measured

• BBa_J23101 (constitutive promoter) + BBa_E0240 (B0032 - RBS, E0040 - GFP, B0015 - double terminator) in pSB1C3
• BBa_J23101 (constitutive promoter) + BBa_E0240 (B0032 - RBS, E0040 - GFP, B0015 - double terminator) in pSB3K3
• BBa_J23115 (constitutive promoter) + BBa_E0240 (B0032 - RBS, E0040 - GFP, B0015 - double terminator) in pSB1C3

Protocols

Sample Preparation

• Inoculate 10 mL of LB with frozen stocks of the three transformed clones (I20260, J23101+E0240, J23115+E0240), a cell background control (TOP10 electrocompetent cells), and an LB only control in a 50 mL Erlenmeyer flask. Grow for 16-18 hours at 37°C, shaking at 300rpm.
• The next morning, add 10µL of each overnight culture to 10 mL of LB with three replicates of each (15 total flasks) and grow 16-18 hours at 37°C, shaking at 300rpm.
• Add 80 µL of each culture to the wells of a clear-bottomed black 96-well plate.

Measurement Protocol

• The samples were plated on a 96-well plate as follows:
• The 96-well plate was inserted into the Infinite 200 PRO Microplate Reader
• Open Tecan i-control, the software used to document the readings
• Configure the settings as detailed above
• Obtain fluorescence and OD600 readings

Sequencing Data