Team:SDU-Denmark/Notebook

From 2014.igem.org

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<tr><td colspan="3"> <h5>Wednesday 18/6 </h5></td></tr>
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The XbaI enzyme didn't work to cut plasmid 161 tuesday and we wanted to find out what was wrong. We had different theories as to what went wrong tuesday:
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<li>Maybe the Xbai didn't have enough time to cut the plasmid. We only gave it 5 minutes.</li>
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<li>Maybe the restriction site on plasmid 161 didn't work.
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</ul>
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To find out which of these theories was right we made three experiments:
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<ol>
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<li>We wanted to do the same as on tuesday, but allow the enzyme to work for 15 minutes instead of 5 minutes, in case this was our mistake.</li>
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<li>We wanted to use the same plasmid as tuesday but cut it with EcoRI to see if this restriction site worked.</li>
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<li>We wanted to try to cut a new plasmid (RFP) with the same enzyme for the same amount of time (5 minutes), to find out if the enzyme worked or not.</li>
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</ol>
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We ran these three experiments and also a control for each of the two plasmids on a PCR, and observed which plasmids where cut. We found out that something was wrong with the X restriction site of plasmid 161, since XbaI didn't cut plasmid 161 (not with 15 minutes either) but did in fact cut RFP. Furthermore we found out that the E restriction site on plasmid 161 worked, since EcoRI was able to cut it.
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Revision as of 09:12, 20 June 2014


Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Lab Journal

Week 25 (16/6 - 22/6)

Monday 16/6

The lab crash course began. We talked a lot about the project goal and the way of getting there. The team is now on the same page and ready for a great summer of exciting and interesting work to begin. In the lab we learned where everything was located and learned how to run a PCR.

- Sarah
Tuesday 17/6

To be written.

Wednesday 18/6
The XbaI enzyme didn't work to cut plasmid 161 tuesday and we wanted to find out what was wrong. We had different theories as to what went wrong tuesday:
  • Maybe the Xbai didn't have enough time to cut the plasmid. We only gave it 5 minutes.
  • Maybe the restriction site on plasmid 161 didn't work.
To find out which of these theories was right we made three experiments:
  1. We wanted to do the same as on tuesday, but allow the enzyme to work for 15 minutes instead of 5 minutes, in case this was our mistake.
  2. We wanted to use the same plasmid as tuesday but cut it with EcoRI to see if this restriction site worked.
  3. We wanted to try to cut a new plasmid (RFP) with the same enzyme for the same amount of time (5 minutes), to find out if the enzyme worked or not.
We ran these three experiments and also a control for each of the two plasmids on a PCR, and observed which plasmids where cut. We found out that something was wrong with the X restriction site of plasmid 161, since XbaI didn't cut plasmid 161 (not with 15 minutes either) but did in fact cut RFP. Furthermore we found out that the E restriction site on plasmid 161 worked, since EcoRI was able to cut it.

To be written.

Thursday 19/6

To be written.

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