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Team:NCTU Formosa/Notebook/test6
From 2014.igem.org
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| + | 2014/9/4(四) | ||
| + | Cultivation of: | ||
| + | Pcons+RBS+AseI PCR product-1,2,3,4,7,8 Ar | ||
| + | XhoI+PSB1K3 PCR product-1,2 Kr | ||
| + | PSB1A3 Ar | ||
| + | Cultivation plate test: | ||
| + | Ar plate and Kr plate | ||
| + | Digestion of: | ||
| + | [PBAN-HindIII] Kr*2 (EP), [PBAN-NheI]Kr*2 (EP), [PBAN-KpnI-HF]Kr*2 (EP) | ||
| + | 23101+B0034+PBAN (HindIII)]Ar(ES),[23101+B0034+PBAN (NheI)]Ar(ES) | ||
| + | PCR of: | ||
| + | [J23101+B0034+KpnI-HF] Ar*3 | ||
| + | Ligation of: | ||
| + | [J23101+B0034+KpnI-HF+PSB1C3] | ||
| + | Electrophoresis of: | ||
| + | [PBAN-HindIII] Kr*2 (EP), [PBAN-NheI]Kr*2 (EP), [PBAN-KpnI-HF]Kr*2 (EP),[23101+B0034+PBAN (HindIII)]Ar(ES),[23101+B0034+PBAN (NheI)]Ar(ES),[23101+B0034+PBAN (HindIII)]Ar(EP)*2,[23101+B0034+PBAN (NheI)]Ar(EP),[23101+B0034+PBAN (KpnI-HF)]Ar(EP)*2,[23101+B0034+PBAN (KpnI-HF)]Ar PCR*3. | ||
| + | </p><p> | ||
| + | 2014/9/5(五) | ||
| + | Electrophoresis: | ||
| + | Well 1 Pcons+RBS+AseI--1 (EP) Ar | ||
| + | Well 2 Pcons+RBS+AseI--2 (EP) Ar | ||
| + | Well 3 Pcons+RBS+AseI--3 (EP) Ar | ||
| + | Well 4 Pcons+RBS+AseI--4 (EP) Ar | ||
| + | Well 5 Pcons+RBS+AseI--7 (EP) Ar （DNA sequencing） | ||
| + | Well 6 Pcons+RBS+AseI--8 (EP) Ar | ||
| + | Well 7 Pcons+RBS+NcoI--2 (EP) Cr （DNA sequencing） | ||
| + | Well 8 Pcons+RBS+Ndel--2 (EP) Cr （DNA sequencing） | ||
| + | Well 9 1 Kb marker | ||
| + | Well 10 Pcons+RBS+Ndel--13 (EP) Cr | ||
| + | Well 11 SacI--3 (XP) Kr | ||
| + | Well 12 SacI--4.1 (XP) Kr | ||
| + | Well 13 SacI--4.2 (XP) Kr | ||
| + | Well 14 XhoI--7 (EP) Kr （DNA sequencing） | ||
| + | Well 15 XhoI--8 (EP) Kr（DNA sequencing） | ||
| + | Well 16 SalI--3 (XP) Kr | ||
| + | Mini prep of: | ||
| + | Pcons+RBS+KpnI-HF-7 | ||
| + | Pcons+RBS+KpnI-HF-8 | ||
| + | Pcons+RBS+KpnI-HF-9 | ||
| + | Digestion of: | ||
| + | Pcons+RBS+KpnI-HF-7 (EP) | ||
| + | Pcons+RBS+KpnI-HF-8 (EP) | ||
| + | Pcons+RBS+KpnI-HF-9 (EP) | ||
| + | |||
| + | Cultivation of: | ||
| + | Pcons+RBS+KpnI-HF Ar PCR product-7,8,9 Ar | ||
| + | Ligation of: | ||
| + | [J23101+B0034+HindIII+B0034+PSB1K3] Kr | ||
| + | [J23101+B0034+HindIII+J61048+PSB1K3] Kr | ||
| + | [J23101+B0034+NheI+B0034+PSB1K3] Kr | ||
| + | [J23101+B0034+NheI+J61048+PSB1K3] Kr | ||
| + | Electrophoresis of digested product: | ||
| + | [Pcons+RBS+KpnI-HF]Ar (EP)*3 | ||
| + | </p> | ||
| + | |||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
Revision as of 14:16, 10 October 2014
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2014/9/4(四) Cultivation of: Pcons+RBS+AseI PCR product-1,2,3,4,7,8 Ar XhoI+PSB1K3 PCR product-1,2 Kr PSB1A3 Ar Cultivation plate test: Ar plate and Kr plate Digestion of: [PBAN-HindIII] Kr*2 (EP), [PBAN-NheI]Kr*2 (EP), [PBAN-KpnI-HF]Kr*2 (EP) 23101+B0034+PBAN (HindIII)]Ar(ES),[23101+B0034+PBAN (NheI)]Ar(ES) PCR of: [J23101+B0034+KpnI-HF] Ar*3 Ligation of: [J23101+B0034+KpnI-HF+PSB1C3] Electrophoresis of: [PBAN-HindIII] Kr*2 (EP), [PBAN-NheI]Kr*2 (EP), [PBAN-KpnI-HF]Kr*2 (EP),[23101+B0034+PBAN (HindIII)]Ar(ES),[23101+B0034+PBAN (NheI)]Ar(ES),[23101+B0034+PBAN (HindIII)]Ar(EP)*2,[23101+B0034+PBAN (NheI)]Ar(EP),[23101+B0034+PBAN (KpnI-HF)]Ar(EP)*2,[23101+B0034+PBAN (KpnI-HF)]Ar PCR*3.
2014/9/5(五) Electrophoresis: Well 1 Pcons+RBS+AseI--1 (EP) Ar Well 2 Pcons+RBS+AseI--2 (EP) Ar Well 3 Pcons+RBS+AseI--3 (EP) Ar Well 4 Pcons+RBS+AseI--4 (EP) Ar Well 5 Pcons+RBS+AseI--7 (EP) Ar （DNA sequencing） Well 6 Pcons+RBS+AseI--8 (EP) Ar Well 7 Pcons+RBS+NcoI--2 (EP) Cr （DNA sequencing） Well 8 Pcons+RBS+Ndel--2 (EP) Cr （DNA sequencing） Well 9 1 Kb marker Well 10 Pcons+RBS+Ndel--13 (EP) Cr Well 11 SacI--3 (XP) Kr Well 12 SacI--4.1 (XP) Kr Well 13 SacI--4.2 (XP) Kr Well 14 XhoI--7 (EP) Kr （DNA sequencing） Well 15 XhoI--8 (EP) Kr（DNA sequencing） Well 16 SalI--3 (XP) Kr Mini prep of: Pcons+RBS+KpnI-HF-7 Pcons+RBS+KpnI-HF-8 Pcons+RBS+KpnI-HF-9 Digestion of: Pcons+RBS+KpnI-HF-7 (EP) Pcons+RBS+KpnI-HF-8 (EP) Pcons+RBS+KpnI-HF-9 (EP) Cultivation of: Pcons+RBS+KpnI-HF Ar PCR product-7,8,9 Ar Ligation of: [J23101+B0034+HindIII+B0034+PSB1K3] Kr [J23101+B0034+HindIII+J61048+PSB1K3] Kr [J23101+B0034+NheI+B0034+PSB1K3] Kr [J23101+B0034+NheI+J61048+PSB1K3] Kr Electrophoresis of digested product: [Pcons+RBS+KpnI-HF]Ar (EP)*3
